UV resonance Raman (UVRR) spectroscopy is a powerful tool for investigating the structure of biological molecules, such as proteins. Numerous UVRR spectroscopic markers that provide information on the structure and environment of the protein backbone and of amino acid side chains have recently been discovered. Combining these UVRR markers with hydrogen-deuterium exchange and advanced statistics is a powerful tool for studying protein systems, including the structure and formation mechanism of protein aggregates and amyloid fibrils. These techniques allow crucial new insights into the structure and dynamics of proteins, such as polyglutamine peptides, which are associated with 10 different neurodegenerative diseases. Here we summarize the spectroscopic structural markers recently developed and the important insights they provide.

中文翻译：

---

用于蛋白质结构和动力学的紫外共振拉曼光谱标记。

紫外线共振拉曼光谱（UVRR）是研究蛋白质等生物分子结构的强大工具。最近发现了许多提供蛋白质主链和氨基酸侧链的结构和环境信息的UVRR光谱标记。将这些UVRR标记与氢-氘交换和先进的统计数据相结合，是研究蛋白质系统（包括蛋白质聚集体和淀粉样蛋白原纤维的结构和形成机理）的强大工具。这些技术使人们能够对与10种不同的神经退行性疾病相关的蛋白质（例如聚谷氨酰胺肽）的结构和动力学提供至关重要的新见解。在这里，我们总结了最近开发的光谱结构标记及其提供的重要见解。