A cytocompatible inkjet bioprinting approach that enables the use of a variety of bioinks to produce hydrogels with a wide range of characteristics is developed. Stabilization of bioinks is caused by horseradish peroxidase (HRP)‐catalyzed cross‐linking consuming hydrogen peroxide (H₂O₂). 3D cell‐laden hydrogels are fabricated by the sequential dropping of a bioink containing polymer(s) cross‐linkable through the enzymatic reaction and H₂O₂ onto droplets of another bioink containing the polymer, HRP, and cells. The ≈95% viability of enclosed mouse fibroblasts and subsequent elongation of the cells in a bioprinted hydrogel consisting of gelatin and hyaluronic acid derivatives suggest the high cytocompatibility of the developed printing approach. The existence of numerous polymers, including derivatives of polysaccharides, proteins, and synthetic polymers, cross‐linkable through the HRP‐catalyzed reaction, means the current approach shows great promise for biofabrication of functional and structurally complex tissues.

中文翻译：

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通过过氧化物酶介导的交联实现滴落式多材料3D生物打印

开发了一种细胞相容性喷墨生物打印方法，该方法能够使用多种生物墨水来生产具有多种特性的水凝胶。生物墨水的稳定是由辣根过氧化物酶（HRP）催化的消耗过氧化氢（H ₂ O ₂）的交联引起的。3D充满细胞的水凝胶是通过依次滴入含有可通过酶促反应和H ₂ O ₂交联的聚合物的生物墨水制成的到另一种含有聚合物，HRP和细胞的生物墨水的液滴上。在由明胶和透明质酸衍生物组成的生物打印水凝胶中，封闭的小鼠成纤维细胞的存活率约为95％，随后细胞伸长，这表明已开发的打印方法具有高度的细胞相容性。通过HRP催化反应可交联的多种聚合物的存在，包括多糖，蛋白质和合成聚合物的衍生物，意味着当前的方法显示出对功能性和结构复杂的组织进行生物制造的巨大希望。