The micro/nanofluidic technique has become an important tool for single-cell analysis with the capability to integrate complex experimental procedures. Micro/nanofluidics-based biochemical analysis exhibits advantages over conventional methods in terms of small sample volume, rapid turnaround time, straightforward operation, and efficient processing. Single-cell manipulation, therapy, detection and sequencing could be implemented within a sophisticated and multi-functional micro/nanofluidic platform. Here we review recent developments of micro/nanofluidic technologies for single-cell analysis, with emphasis on cell trapping, treatment, and biochemical studies. The potential of micro/nanofluidics-based single-cell analysis is discussed.

微/纳流体技术已成为具有集成复杂实验程序能力的单细胞分析的重要工具。基于微/纳米流体的生化分析与传统方法相比，在小样品量，快速周转时间，直接操作和高效处理方面显示出优势。单细胞操作，治疗，检测和测序可在复杂的多功能微/纳流体平台内实施。在这里，我们回顾了用于单细胞分析的微/纳米流体技术的最新发展，重点是细胞捕获，治疗和生化研究。讨论了基于微/纳米流体的单细胞分析的潜力。