Hydrogen sulfide (H₂S) and biothiol molecules, such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), play an important role in biology. However, understanding the complicated relationship between H₂S and biothiols remains an enormous challenge owing to the difficulty in sensing H₂S and biothiols simultaneously. Therefore, the development of probes for detecting H₂S and biothiols is of great importance in biological science. In this work, we reported a novel fluorescent probe for the sensitive and selective detection of H₂S and glutathione (GSH) simultaneously in different buffer solutions. The key design principle is based on a coumarin as the fluorophore structuring a fluorescent probe with three potential sites which could react with H₂S and biothiols. This probe displays a rapid response with highly sensitive and selective detection of H₂S and GSH (the detection limit of 75 nM and 280 nM, respectively). Moreover, with the assistance of a confocal fluorescence microscope, we demonstrated that the probe can be successfully applied for imaging H₂S and GSH in MCF-7 cells.

中文翻译：

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同时检测硫化氢和谷胱甘肽的三位荧光探针及其生物成像应用†

硫化氢（H ₂ S）和生物硫醇分子，例如谷胱甘肽（GSH），半胱氨酸（Cys）和高半胱氨酸（Hcy）在生物学中起重要作用。然而，由于难以同时感测H ₂ S和生物硫醇，因此理解H ₂ S和生物硫醇之间的复杂关系仍然是巨大的挑战。因此，开发用于检测H ₂ S和生物硫醇的探针在生物科学中具有重要意义。在这项工作中，我们报道了一种用于H ₂灵敏和选择性检测的新型荧光探针S和谷胱甘肽（GSH）同时在不同的缓冲溶液中。关键的设计原理是基于香豆素作为荧光基团，该荧光基团构成了一个具有三个可能与H ₂ S和生物硫醇反应的潜在位点的荧光探针。该探针显示出对H ₂ S和GSH的高灵敏度和选择性检测（分别为75 nM和280 nM的检测极限）的快速响应。此外，借助共聚焦荧光显微镜，我们证明了该探针可成功用于MCF-7细胞中的H ₂ S和GSH成像。