A cancer immunotherapy μ-environment LabChip, equipped with titanium oxide phthalocyanine (TiOPc)-based optoelectronic tweezers (OET) to achieve direct cell–cell contact, can be used to study the interaction between immune cells and other cells for real-time analysis of NK cells' behavior. In microfluidic devices, it is difficult to solve dead zone problems and observe dynamic cell–cell interactions. We have created a stable and static culture μ-environment which can enhance NK cell activities. In addition, OET is used to solve dead zone problems by manipulating a single cell into four-leaf-clover-shaped (FLCS) microwells made of poly(ethylene glycol) diacrylate (PEG-DA) through optofluidic maskless lithography, causing direct cell–cell contact. Our design reconstructed an in vitro human immune system for the study of dynamic immunological response. When the NK cells came into contact with the target cells in the μ-environment LabChip, we observed that the target cells showed apoptotic characteristics (i.e. cell shrinkage and blebbing within 2 h and then die within 3 h). In addition, our μ-environment LabChip demonstrated higher NK cell activity compared with conventional analysis. We have created an innovative cancer immunotherapy μ-environment LabChip to provide a stable and static μ-environment for cell–cell interaction study. Furthermore, our μ-environment LabChip showed the potential to enhance NK cell activity and to study immunological interactions between immune cells and cancer cells dynamically.

中文翻译：

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癌症免疫疗法μ环境LabChip：利用光电镊子†

配备基于二氧化钛酞菁（TiOPc）的光电镊子（OET）的癌症免疫疗法μ环境LabChip可用于研究细胞与细胞之间的直接接触，可用于研究免疫细胞与其他细胞之间的相互作用，从而进行实时分析。 NK细胞的行为。在微流体设备中，很难解决死区问题并观察动态的细胞间相互作用。我们已经创建了可以增强NK细胞活性的稳定和静态的培养μ环境。此外，OET还可以通过将单个细胞通过光流体无掩模光刻处理成由聚乙二醇二丙烯酸酯（PEG-DA）制成的四叶三叶草形（FLCS）微孔来解决死区问题，从而导致直接细胞–细胞接触。我们的设计重建了体外人体免疫系统用于动态免疫反应的研究。当NK细胞与μ环境LabChip中的靶细胞接触时，我们观察到靶细胞显示出凋亡特征（即，细胞在2小时内收缩和起泡，然后在3小时内死亡）。此外，与传统分析相比，我们的μ环境LabChip具有更高的NK细胞活性。我们创建了一种创新的癌症免疫疗法μ环境LabChip，可为细胞间相互作用研究提供稳定和静态的μ环境。此外，我们的μ环境LabChip具有增强NK细胞活性并动态研究免疫细胞与癌细胞之间的免疫相互作用的潜力。