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Macrophage responses to the physical burden of cell-sized particles†
Journal of Materials Chemistry B ( IF 7 ) Pub Date : 2017-12-04 00:00:00 , DOI: 10.1039/c7tb01673e
Hua Yue 1, 2, 3, 4, 5 , Lan Yuan 5, 6, 7 , Weiwei Zhang 5, 8, 9, 10 , Shujia Zhang 5, 8, 9, 10 , Wei Wei 1, 2, 3, 4, 5 , Guanghui Ma 1, 2, 3, 4, 5
Affiliation  

The role of a biophysical signal on cell response has excited tremendous interest recently. Herein, we exploited an “intake method” together with uniform autofluorescent cell-sized particles to investigate macrophage responses against different particle burdens. Our work not only revealed an insatiable macrophage uptake of cell-sized microparticles (MPs) but also widened the theoretical size and volume range for particle entry. MPs outperform NPs in the utmost volume of intracellular particles, indicating a converse size-associated event than originally anticipated. Such a superior volume burden (2.64-fold of the cell volume) for MPs is highly correlated to less membrane loss (19-fold below that for NPs) and more evident deformation of the cell membrane/nucleus. In addition, the cells with a high burden of MPs exhibit moderate migration activity, which is in line with mild cytokine release. These results highlight the indispensable role of physical burden on the regulation of macrophage functions, providing important views for desired biological outcomes and minimal side effects.

中文翻译:

巨噬细胞对细胞大小颗粒的物理负担的反应

近来,生物物理信号在细胞反应中的作用引起了极大的兴趣。在本文中,我们利用“摄入方法”以及均匀的自发荧光细胞大小的颗粒来研究巨噬细胞对不同颗粒负荷的反应。我们的工作不仅揭示了细胞大小的微粒(MPs)不能满足的巨噬细胞摄取,而且还拓宽了进入粒子的理论尺寸和体积范围。MPs在最大体积的细胞内颗粒中胜过NPs,这表明与尺寸相关的事件发生了逆转,超出了最初的预期。MP的这种优越的体积负担(细胞体积的2.64倍)与较少的膜损失(比NP的膜损失低19倍)和细胞膜/细胞核更明显的变形高度相关。此外,MP负担高的细胞表现出中等的迁移活性,这与细胞因子的轻度释放是一致的。这些结果突出了身体负担对巨噬细胞功能调节的不可或缺的作用,为所需的生物学结果和最小的副作用提供了重要的观点。
更新日期:2017-12-04
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