Microarray technology provides an excellent platform for biomedical and biochemical research including basic scientific studies, drug discovery, and diagnostics. Here, we develop a novel method referred to as real-time two-photon lithography in a controlled flow in which femtosecond laser two-photon lithography is performed in situ in the sequential mode stopping and flowing the flow of liquid resin containing microparticles to achieve 100% trapping on a one-bead-to-one-trap basis. Polydisperse particles can be all trapped to form a desired array by freely designing trap structures, resulting in an unprecedentedly high capture efficiency of ∼100%. No persistent pressure is needed after trapping which reduces the complexity of the system. In addition, trapping of particle-cluster arrays with a controlled number of particles is also achieved via this method. The trapped particles inside the microchip are successfully applied as microlenses for high quality imaging. The present technology marks an essential step towards a versatile platform for the integration of bead-based assays and paves the way for developing innovative microfluidics, optofluidics, micro-optics and single-cell analysis devices.

中文翻译：

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实时双光子光刻技术在受控流中创建用于光流成像的单微粒阵列和微粒簇阵列†

微阵列技术为生物医学和生化研究（包括基础科学研究，药物发现和诊断）提供了一个极好的平台。在这里，我们开发了一种在受控流中进行实时二光子光刻的新颖方法，在该流中原位执行了飞秒激光二光子光刻在连续模式下，停止含微粒的液态树脂的流动并使之流动，以实现一对一捕集的100％捕集。通过自由设计捕集结构，可以将多分散颗粒全部捕集以形成所需的阵列，从而获得约100％的空前高捕获效率。捕集后无需持续施加压力，从而降低了系统的复杂性。此外，还可以通过以下方式捕获具有受控数量的粒子的粒子集群阵列：这种方法。微芯片内部捕获的粒子已成功地用作微透镜，用于高质量成像。本技术标志着迈向集成基于微珠的检测的通用平台的重要一步，并为开发创新的微流体，光流体，微光学和单细胞分析设备铺平了道路。