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Programmable Peptide-Cross-Linked Nucleic Acid Nanocapsules as a Modular Platform for Enzyme Specific Cargo Release
Bioconjugate Chemistry ( IF 4.7 ) Pub Date : 2017-11-30 00:00:00 , DOI: 10.1021/acs.bioconjchem.7b00629
Joshua J. Santiana 1 , Binglin Sui 1 , Nicole Gomez 1 , Jessica L. Rouge 1
Affiliation  

Herein we describe a modular assembly strategy for photo-cross-linking peptides into nucleic acid functionalized nanocapsules. The peptides embedded within the nanocapsules form discrete nanoscale populations capable of gating the release of molecular and nanoscale cargo using enzyme–substrate recognition as a triggered release mechanism. Using photocatalyzed thiol–yne chemistry, different peptide cross-linkers were effectively incorporated into the nanocapsules and screened against different proteases to test for degradation specificity both in vitro and in cell culture. By using a combination of fluorescence assays, confocal and TEM microscopy, the particles were shown to be highly specific for their enzyme targets, even between enzymes of similar protease classes. The rapid and modular nature of the assembly strategy has the potential to be applied to both intracellular and extracellular biosensing and drug delivery applications.

中文翻译:

可编程肽交联核酸纳米胶囊作为酶特异性货物释放的模块化平台

在本文中,我们描述了将肽光交联成核酸功能化的纳米胶囊的模块化组装策略。嵌入纳米胶囊中的肽形成离散的纳米级种群,能够通过酶底物识别作为触发释放机制来控制分子和纳米级货物的释放。使用光催化的硫醇-炔化学,可以将不同的肽交联剂有效地掺入纳米胶囊中,并针对不同的蛋白酶进行筛选,以在体外测试降解特异性和细胞培养。通过结合使用荧光测定,共聚焦和TEM显微镜,即使对相似蛋白酶类别的酶,颗粒也显示出对其酶标靶的高度特异性。组装策略的快速和模块化性质具有被应用于细胞内和细胞外生物传感和药物递送应用的潜力。
更新日期:2017-11-30
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