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In vitro assessment of sex steroids and related compounds in water and sediments – a critical review
Environmental Science: Processes & Impacts ( IF 5.5 ) Pub Date : 2017-11-29 00:00:00 , DOI: 10.1039/c7em00458c
Chimi Wangmo 1, 2, 3, 4 , Sergio Jarque 1, 2, 3, 4 , Klára Hilscherová 1, 2, 3, 4 , Luděk Bláha 1, 2, 3, 4 , Michal Bittner 1, 2, 3, 4
Affiliation  

Detection of endocrine disrupting compounds in water and sediment samples has gained much importance since the evidence of their effects was reported in aquatic ecosystems in the 1990s. The aim of this review is to highlight the advances made in the field of in vitro analysis for the detection of hormonally active compounds with estrogenic, androgenic and progestogenic effects in water and sediment samples. In vitro assays have been developed from yeast, mammalian and in a few cases from fish cells. These assays are based either on the hormone-mediated proliferation of sensitive cell lines or on the hormone-mediated expression of reporter genes. In vitro assays in combination with various sample enrichment methods have been used with limits of detection as low as 0.0027 ng L−1 in water, and 0.0026 ng g−1 in sediments for estrogenicity, 0.1 ng L−1 in water, and 0.5 ng g−1 in sediments for androgenicity, and 5 ng L−1 in water for progestogenicity expressed as equivalent concentrations of standard reference compounds of 17β-estradiol, dihydrotestosterone and progesterone, respectively. The experimental results and limits of quantification, however, are influenced by the methods of sample collection, preparation, and individual laboratory practices.

中文翻译:

水和沉积物中性类固醇和相关化合物的体外评估-一项重要评论

自1990年代以来在水生生态系统中报告了其影响的证据以来,对水和沉积物样品中内分泌干扰化合物的检测就变得非常重要。这篇综述的目的是强调在体外分析领域中检测水和沉积物样品中具有雌激素,雄激素和孕激素作用的激素活性化合物方面取得的进展。已经从酵母,哺乳动物以及在少数情况下从鱼细胞开发了体外测定法。这些测定是基于激素介导的敏感细胞系增殖或基于激素介导的报告基因表达。体外与各种样品富集方法组合测定法已被用于检测限低至0.0027纳克大号-1在水中,并且0.0026纳克克-1沉积物为雌激素,0.1纳克大号-1在水和0.5纳克克- 1沉积物为androgenicity,和5ng大号-1在水中表示为分别的17β雌二醇,二氢和孕酮,标准参照化合物当量浓度progestogenicity。但是,实验结果和定量极限受样品收集,制备和各个实验室操作方法的影响。
更新日期:2017-11-29
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