Bioconjugates based on a redox protein and iron oxide magnetic nanoparticles were employed in the catalytic polymerization of ortho-, meta- and para-substituted phenylenediamines at room temperature for the synthesis of carbon-based fluorescent polymers. UV-Vis absorption measurements of the three obtained products showed a red shift compared to the starting materials. These results together with the FT-IR and XPS analyses confirm the successful formation of the polymers. In particular, the component quantification in the C 1s XPS spectra revealed the high proportion of C–N bonds, associated with the oxidative polymerization of the precursors. MALDI-TOF MS analysis was performed in order to determine the molecular weights of the products. The synthesized poly-oPDA, poly-mPDA and poly-pPDA resulted to have a highly green, blue and red fluorescence, respectively. The reusability of the biocatalyst and the effect of the pH were investigated in the reaction for the ortho isomer. The biocatalytic system showed optimum results when the pH was below the enzyme isoelectric point (pI).

中文翻译：

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催化活性磁性生物共轭物驱动的碳基荧光聚合物的合成

在的催化聚合采用了基于氧化还原蛋白和氧化铁磁性纳米粒子的生物缀合物的邻- ，间位-和对位取代的苯二胺在室温下用于合成碳基荧光聚合物。与起始材料相比，三种获得的产物的UV-Vis吸收测量显示出红移。这些结果与FT-IR和XPS分析一起证实了聚合物的成功形成。尤其是，C 1s XPS光谱中的组分定量揭示了高比例的C–N键，这与前体的氧化聚合有关。为了确定产物的分子量，进行了MALDI-TOF MS分析。将合成的聚Ô PDA，聚米PDA和聚pPDA分别具有高度绿色，蓝色和红色荧光。在邻位异构体的反应中研究了生物催化剂的可重复使用性和pH的影响。当pH值低于酶等电点（pI）时，生物催化系统显示出最佳结果。