2-Deoxy-d-ribose-5-phosphate aldolase (DERA) is a biocatalyst that is capable of converting acetaldehyde and a second aldehyde as acceptor into enantiomerically pure mono- and diyhydroxyaldehydes, which are important structural motifs in a number of pharmaceutically active compounds. However, substrate as well as product inhibition requires a more-sophisticated process design for the synthesis of these motifs. One way to do so is to the couple aldehyde conversion with transport processes, which, in turn, would require an immobilization of the enzyme within a thin film that can be deposited on a membrane support. Consequently, we developed a fabrication process for such films that is based on the formation of DERA–poly(N-isopropylacrylamide) conjugates that are subsequently allowed to self-assemble at an air–water interface to yield the respective film. In this contribution, we discuss the conjugation conditions, investigate the interfacial properties of the conjugates, and, finally, demonstrate a successful film formation under the preservation of enzymatic activity.

中文翻译：

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通过2-脱氧-d-核糖-5-磷酸醛缩酶-聚（N-异丙基丙烯酰胺）自组装的生物催化活性薄膜

2-脱氧ð核糖-5-磷酸醛缩酶（DERA）是生物催化剂，其能够将乙醛和第二醛作为受体进入对映体纯的单-和diyhydroxyaldehydes，这是很重要的结构基序在许多药物活性化合物的。然而，底物以及产物的抑制需要更复杂的工艺设计来合成这些基序。这样做的一种方法是将醛的转化与运输过程耦合在一起，而运输过程又需要将酶固定在可以沉积在膜载体上的薄膜内。因此，我们基于DERA-poly（N-异丙基丙烯酰胺）共轭物，随后允许其在空气-水界面处自组装以产生相应的膜。在这一贡献中，我们讨论了缀合条件，研究了缀合物的界面性质，最后证明了在酶活性保持下的成功成膜。