Glyphosate (N-(phosphonomethyl)glycine) is the most widely used herbicide on earth. A simple assay to quantify glyphosate concentrations in environmental samples was developed as part of an interdisciplinary effort linking introductory laboratory courses in chemistry, biology, and microbiology. In this 3 h laboratory experiment, students used UV–vis spectroscopy to quantify glyphosate in prepared unknowns and supernatants from glyphosate-treated soil samples. Regression analysis indicated that the assay is linear up to 20.0 ppm, making it particularly useful for detection of low levels of glyphosate in environmental samples. The assay can be used to quantify the activity of glyphosate-degrading soil microorganisms by comparing glyphosate levels between autoclaved and nonautoclaved soil slurries.

中文翻译：

---

跨学科本科实验室草甘膦的紫外可见分光光度分析和定量

草甘膦（N-（膦酰基甲基）甘氨酸）是地球上使用最广泛的除草剂。作为跨学科工作的一部分，开发了一种简单的测定方法来量化环境样品中的草甘膦浓度，该工作将化学，生物学和微生物学入门实验室课程联系在一起。在这个3小时的实验室实验中，学生使用紫外-可见光谱法对草甘膦处理过的土壤样品中准备好的未知物和上清液中的草甘膦进行了定量。回归分析表明，该测定法线性分析高达20.0 ppm，使其特别适用于检测环境样品中的低水平草甘膦。通过比较高压灭菌和非高压灭菌的土壤浆液中的草甘膦含量，该测定法可用于定量降解草甘膦的土壤微生物的活性。