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Increases in the competitive fitness of West Nile virus isolates after introduction into California.
Virology ( IF 3.7 ) Pub Date : 2017-11-28 , DOI: 10.1016/j.virol.2017.11.017
Gabriella Worwa 1 , Andra A Hutton 1 , Michèle Frey 1 , Nisha K Duggal 2 , Aaron C Brault 2 , William K Reisen 1
Affiliation  

To investigate the phenotypic evolution of West Nile virus (WNV) in California, we competed sixteen isolates made during 2007–08 against COAV997-5nt, a genetically marked clone from the founding 2003 California isolate COAV997-2003. Using in vivo fitness competitions in House Finches (HOFI) and Culex tarsalis mosquitoes, we found that the majority of WNV WN02 and SW03 genotype isolates exhibited elevated replicative fitness in both hosts compared to COAV997-5nt. Increased replicative capacity in HOFIs was not associated with increased mortality, indicating that these isolates had not gained avian virulence. One WN02 isolate from Coachella Valley, a region geographically close to the isolation of COAV997, showed neutral fitness in HOFIs and reduced fitness in Cx. tarsalis. Two isolates from Kern County and Sacramento/Yolo County out-competed COAV997-nt in HOFIs, but were transmitted less efficiently by Cx. tarsalis. Competition demonstrated neutral or increased fitness that appeared independent of both WN02 and SW03 genotypes.



中文翻译:

引入加利福尼亚后,西尼罗河病毒分离株的竞争适应性增强。

为了研究西尼罗河病毒(WNV)在加利福尼亚州的表型演变,我们与2007-08年间制得的16种分离株竞争了COAV997-5nt,COAV997-5nt是来自始建于2003年的加利福尼亚分离株COAV997-2003的基因标记克隆。使用家雀(HOFI)和Culex tarsalis mosquitoes)的体内适应性竞争,我们发现与WAV997-5nt相比,大多数WNV WNO2和SW03基因型分离株在两个宿主中均表现出较高的复制适应性。HOFIs复制能力增强与死亡率增加无关,表明这些分离株未获得禽毒力。来自Coachella谷的一个WN02分离株在地理上接近COAV997的分离区,在HOFIs中显示中性适应,而在Cx中显示降低s。来自克恩县和萨克拉曼多/尤洛县的两个分离株在HOFI中胜过COAV997-nt,但被Cx传播的效率较低s。竞争表明,中性或增强的适应性与WN02和SW03基因型无关。

更新日期:2017-11-28
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