Construction of a new multifunctional chemo/biosensing platform for small biomolecules and tumor markers is of great importance in analytical chemistry. Herein, a novel universal multifunctional nanoplatform for biomolecules and enzyme activity detection was proposed based on fluorescence resonance energy transfer (FRET) between upconversion nanoparticles (UCNPs) and target-inducing enlarged gold nanoparticles (AuNPs). The reductive molecule such as H₂O₂ can act as the reductant to reduce HAuCl₄, which will make the Au seeds grow. The enlarged AuNPs can effectively quench the fluorescence of UCNPs owing to the good spectral overlap between the absorption band of the AuNPs and the emission band of the UCNPs. Utilizing the FRET between the UCNPs and enlarged AuNPs, good linear relationship between the fluorescence of UCNPs and the concentration of H₂O₂ can be found. Based on this strategy, H₂O₂ related molecules such as l-lactate, glucose, and uric acid can also be quantified. On the basis of UCNPs and PVP/HAuCl₄, a general strategy for other reductants such as ascorbic acid (AA), dopamine (DA), or enzyme activity can be established. Therefore, the universal multifunctional nanoplatform based on UCNPs and the target-inducing in situ enlarged Au NPs will show its potential as a simple method for the detection of some life related reductive molecules, enzyme substrates, as well as enzyme activity.

中文翻译：

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基于靶诱导原位基因的通用多功能纳米平台促进金种子的生长以抑制上转换纳米粒子的荧光

构建用于小生物分子和肿瘤标志物的新型多功能化学/生物传感平台在分析化学中具有重要意义。本文中，基于上转换纳米粒子（UCNPs）和诱导靶标的扩大化金纳米粒子（AuNPs）之间的荧光共振能量转移（FRET），提出了一种用于生物分子和酶活性检测的新型通用多功能纳米平台。H ₂ O ₂等还原性分子可作为还原剂还原HAuCl ₄，这将使金种子生长。由于AuNPs的吸收带和UCNPs的发射带之间具有良好的光谱重叠，因此增大的AuNPs可以有效地猝灭UCNPs的荧光。利用UCNPs和扩大的AuNPs之间的FRET，可以发现UCNPs的荧光与H ₂ O ₂的浓度之间具有良好的线性关系。基于该策略，还可以定量H ₂ O ₂相关分子，例如l-乳酸，葡萄糖和尿酸。基于UCNP和PVP / HAuCl ₄，可以建立其他还原剂（如抗坏血酸（AA），多巴胺（DA）或酶活性）的一般策略。因此，基于UCNPs和目标诱导原位扩增的Au NPs的通用多功能纳米平台将显示其作为检测某些与生命有关的还原分子，酶底物以及酶活性的简单方法的潜力。