The identification of molecular tags along nucleic acid sequences has many potential applications in bionanotechnology, disease biomarker detection, and DNA sequencing. An attractive approach to this end is the use of solid-state nanopores, which can electrically detect molecular substructure and can be integrated into portable lab-on-a-chip sensors. We present here a DNA origami-based approach of molecular assembly in which solid-state nanopores are capable of differentiating 165 bp scaffolds containing zero, one, and two dsDNA protrusions. This highly scalable technique requires minimal sample preparation and is customizable for a wide range of targets and applications. As a proof-of-concept, an aptamer-based DNA displacement reaction is performed in which a dsDNA protrusion is formed along a 255 bp scaffold in the presence of ATP. While ATP is too small to be directly sensed using conventional nanopore methods, our approach allows us to detect ATP by identifying molecular substructure along the DNA scaffold.

中文翻译：

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使用固态纳米孔鉴定短DNA支架中的结构。

沿核酸序列鉴定分子标签在仿生技术，疾病生物标志物检测和DNA测序中具有许多潜在应用。为此目的，一种有吸引力的方法是使用固态纳米孔，它可以电检测分子的亚结构，并且可以集成到便携式芯片实验室传感器中。我们在这里介绍一种基于DNA折纸的分子组装方法，其中固态纳米孔能够区分包含零，一个和两个dsDNA突起的165 bp支架。这种高度可扩展的技术需要最少的样品制备，并且可针对各种目标和应用进行定制。作为概念证明，进行基于适体的DNA置换反应，其中在存在ATP的情况下沿着255bp的支架形成dsDNA突起。