A novel zwitterionic polymer ionic liquid functionalized magnetic nanospheres, shortly as Fe₃O₄@PCL-PILs, is synthesized by grafting ionic liquid VimCOOHBr onto polymer ε-caprolactone (PCL) modified magnetic nanospheres via esterification and surface-initiated free radical polymerization. This established synthesis strategy offers the obtained magnetic nanospheres with well-defined core–corona structure, compact grafting layer, favorable zwitterionic and negative-charged surface, and high magnetic susceptibility. The as-prepared Fe₃O₄@PCL-PILs nanospheres exhibit typical “zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)” behaviors toward protein binding, and selectively adsorption of glycoprotein is achieved. The adsorption capacity of the magnetic nanospheres toward Immunoglobulin G is high up to 1136.4 mg g^–1, and the captured Immunoglobulin G could be efficiently recovered by using 0.5% NH₃ H₂O (v/v) as stripping reagent, providing a recovery of 80.5%. Fe₃O₄@PCL-PILs nanospheres are then employed as sorbent for the selective isolation of Immunoglobulin G from human whole blood, obtaining high-purity Immunoglobulin G as demonstrated by polyacrylamide gel electrophoresis assays.

中文翻译：

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两性离子聚合物离子液体功能化的核心-电晕磁性纳米球，用于糖蛋白的高度选择性分离

通过酯化和表面引发的自由基聚合将离子液体VimCOOHBr接枝到聚合物ε-己内酯（PCL）修饰的磁性纳米球上，合成了一种新型的两性离子聚合物离子液体功能化的磁性纳米球，简称为Fe ₃ O ₄ @ PCL-PILs。这种既定的合成策略为获得的磁性纳米球提供了明确的核电晕结构，致密的接枝层，良好的两性离子和带负电荷的表面以及高磁化率。制备的Fe ₃ O ₄@ PCL-PILs纳米球表现出典型的“两性离子亲水相互作用液相色谱（ZIC-HILIC）”对蛋白质结合的行为，并实现了糖蛋白的选择性吸附。磁性纳米球对免疫球蛋白G的吸附能力高达1136.4 mg g ^–1，并且通过使用0.5％NH ₃ H ₂ O（v / v）作为汽提剂可以有效地回收捕获的免疫球蛋白G ，从而提供回收率80.5％。然后将Fe ₃ O ₄ @ PCL-PILs纳米球用作吸附剂，用于从人全血中选择性分离免疫球蛋白G，从而获得高纯度的免疫球蛋白G，这已通过聚丙烯酰胺凝胶电泳分析证实。