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Identification of Mature Atherosclerotic Plaque Proteome Signatures Using Data-Independent Acquisition Mass Spectrometry
Journal of Proteome Research ( IF 4.4 ) Pub Date : 2017-11-28 00:00:00 , DOI: 10.1021/acs.jproteome.7b00487
Nicole Hansmeier 1 , Josef Buttigieg 2 , Pankaj Kumar 2 , Shaneen Pelle 2 , Kyoo Yoon Choi 2 , David Kopriva 3 , Tzu-Chiao Chao 2
Affiliation  

Atherosclerosis is a chronic inflammatory disease with complex pathobiology and one of the most common causes of cardiovascular events. The process is characterized by complex vascular remodeling processes that require the actions of numerous proteins. The composition of atherosclerotic plaque is increasingly recognized as a major factor governing the occurrence of cardiovascular or neurological symptoms. To gain deeper insights into the composition of atherosclerotic plaques, we created quantitative proteome profiles of advanced plaque tissues of six male patients undergoing carotid endarterectomy for stroke prevention. Using a quantitative, data-independent proteome approach, we identified 4181 proteins with an average protein coverage of 45%. An analysis of the quantitative composition of the tissue revealed key players of vascular remodeling processes. Moreover, compared with proximal arterial tissue, 20 proteins in mature plaques were enriched, whereas 52 proteins were found in lower quantities. Among the proteins with increased abundance were prominent extracellular matrix proteins such as biglycan and lumican, whereas cytoskeletal markers for contractile smooth muscle cells (SMCs) were decreased. Taken together, this study provides the most comprehensive quantitative assessment of mature human plaque tissue to date, which indicates a central role of SMCs in the structure of advanced atherosclerotic plaques.

中文翻译:

使用独立于数据的采集质谱法鉴定成熟的动脉粥样硬化斑块蛋白质组特征

动脉粥样硬化是一种慢性炎症性疾病,具有复杂的病理生物学特性,是心血管事件的最常见原因之一。该过程的特征是复杂的血管重塑过程,需要大量蛋白质的作用。动脉粥样硬化斑块的组成日益被认为是控制心血管或神经系统症状发生的主要因素。为了更深入地了解动脉粥样硬化斑块的组成,我们创建了六名接受颈动脉内膜切除术以预防中风的男性患者的晚期斑块组织的定量蛋白质组学分析。使用定量的,不依赖数据的蛋白质组学方法,我们鉴定了4181种蛋白质,平均蛋白质覆盖率为45%。对组织的定量组成的分析揭示了血管重塑过程的关键因素。此外,与近端动脉组织相比,成熟斑块中的20种蛋白质富集,而52种蛋白质的含量较低。在丰度增加的蛋白质中,突出的细胞外基质蛋白质例如双糖链蛋白聚糖和卢米肯聚糖,而收缩性平滑肌细胞(SMCs)的细胞骨架标志物则减少。综上所述,这项研究提供了迄今为止最成熟的人类斑块组织的最全面的定量评估,这表明了SMC在晚期动脉粥样硬化斑块结构中的核心作用。在丰度增加的蛋白质中,突出的细胞外基质蛋白质例如双糖链蛋白聚糖和卢米肯聚糖,而收缩性平滑肌细胞(SMCs)的细胞骨架标志物则减少。综上所述,这项研究提供了迄今为止最成熟的人类斑块组织的最全面的定量评估,这表明了SMC在晚期动脉粥样硬化斑块结构中的核心作用。在丰度增加的蛋白质中,突出的细胞外基质蛋白质例如双糖链蛋白聚糖和卢米肯聚糖,而收缩性平滑肌细胞(SMCs)的细胞骨架标志物则减少。综上所述,这项研究提供了迄今为止最成熟的人类斑块组织的最全面的定量评估,这表明了SMC在晚期动脉粥样硬化斑块结构中的核心作用。
更新日期:2017-11-28
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