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Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings
Nature Structural & Molecular Biology ( IF 16.8 ) Pub Date : 2017-11-27 , DOI: 10.1038/s41594-017-0001-9
Shotaro Otsuka , Anna M. Steyer , Martin Schorb , Jean-Karim Hériché , M. Julius Hossain , Suruchi Sethi , Moritz Kueblbeck , Yannick Schwab , Martin Beck , Jan Ellenberg

The nuclear envelope has to be reformed after mitosis to create viable daughter cells with closed nuclei. How membrane sealing of DNA and assembly of nuclear pore complexes (NPCs) are achieved and coordinated is poorly understood. Here, we reconstructed nuclear membrane topology and the structures of assembling NPCs in a correlative 3D EM time course of dividing human cells. Our quantitative ultrastructural analysis shows that nuclear membranes form from highly fenestrated ER sheets whose holes progressively shrink. NPC precursors are found in small membrane holes and dilate radially during assembly of the inner ring complex, forming thousands of transport channels within minutes. This mechanism is fundamentally different from that of interphase NPC assembly and explains how mitotic cells can rapidly establish a closed nuclear compartment while making it transport competent.

中文翻译:

有丝分裂后核孔的组装通过小膜孔的径向扩张而进行

有丝分裂后必须对核被膜进行改造,以形成具有闭合核的存活子细胞。人们对如何实现DNA膜的密封和核孔复合体(NPC)的组装和协调的了解甚少。在这里,我们在分裂人类细胞的相关3D EM时间过程中重建了核膜拓扑结构和NPC的组装结构。我们的定量超微结构分析表明,核膜由高度开孔的ER片形成,其孔逐渐收缩。NPC前体存在于小的膜孔中,在内环配合物组装过程中呈放射状扩张,在数分钟内形成数千个运输通道。
更新日期:2017-11-28
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