Trends in Food Science & Technology ( IF 15.3 ) Pub Date : 2017-11-23 , DOI: 10.1016/j.tifs.2017.11.014 Aiping Liu , Laura Anfossi , Li Shen , Cheng Li , Xiaohong Wang
Background
Immunoassays have gained considerable attention in safety assurance for food, feed and agricultural products. Generally, immunoassays are presented either in a competitive or non-competitive, sandwich-type format, and the former is extensively employed for low-molecular-weight contaminants, which usually bear one accessible epitope. Theoretically, non-competitive, sandwich-type immunoassays have higher sensitivity, precision and linearity. However, the analyte to be measured in such a format must be large enough to have at least two epitopes to be captured. It is not feasible to detect low-molecular-weight contaminants through conventional non-competitive sandwich-type immunoassay. Consequently, there is a trend to develop new types of sensitive non-competitive immunoassays for low-molecular-weight contaminants.
Scope and approach
This article reviews the progress in non-competitive immunoassays for low molecular weight contaminants in food, feed and agricultural products, including the principles, applications and suggested perspectives for this field.
Key findings and conclusions
Anti-metatype antibody-based immunoassays are the most promising method, but dissociation of the antibody-hapten complex might be a challenge, and therefore more in-depth research should be focused on preparation of new formats of the antibody-hapten complex. Meanwhile, strategies for direct non-competitive detection or aimed at the simultaneous detection of different targets would be especially desirable besides focusing on improving the sensitivity and specificity of the detection.
中文翻译:
用于食品,饲料和农产品中低分子量污染物检测的非竞争性免疫测定:简要评述
背景
免疫分析在食品,饲料和农产品的安全保证方面已引起了广泛关注。通常,免疫分析以竞争性或非竞争性的夹心型形式出现,前者广泛用于低分子量污染物,通常带有一个可接近的表位。从理论上讲,非竞争性夹心型免疫测定具有更高的灵敏度,精度和线性。然而,以这种形式被测量的分析物必须足够大以具有至少两个要被捕获的表位。通过常规的非竞争性夹心型免疫测定法检测低分子量污染物是不可行的。因此,存在针对低分子量污染物开发新型的敏感的非竞争性免疫测定的趋势。
范围和方法
本文回顾了食品,饲料和农产品中低分子量污染物的非竞争性免疫分析方法的进展,包括该领域的原理,应用和建议的观点。
主要发现和结论
基于抗metatype抗体的免疫测定法是最有前途的方法,但是抗体-半抗原复合物的解离可能是一个挑战,因此,更深入的研究应该集中在抗体-半抗原复合物新形式的制备上。同时,除了专注于提高检测的灵敏度和特异性之外,直接非竞争性检测或旨在同时检测不同靶标的策略将是特别期望的。