A new water soluble and fluorogenic probe (L) that can demonstrate a specific ratiometric detection of a SO₂ derivative (SO₃²⁻) in 100% aqueous medium and live cells has been designed and synthesized. The detection process can be visualized by the naked eye, as the orange-red fluorescence of L turns into a strong blue fluorescence upon interaction with SO₃²⁻. L displayed several beneficial attributes such as detection in complete aqueous medium, extremely fast response time along with high selectivity and sensitivity. The ratiometric sensing was attributed to the selective nucleophilic addition reaction of SO₃²⁻ with L. The probe was further used to develop a low cost microfluidic sensor device (μPAD). The probe was biocompatible and its potential to sense SO₃²⁻ in mitochondria was captured in live HeLa cells.

中文翻译：

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用于实时检测水性介质中SO ₃ ^2-的比例荧光探针：在基于纤维素纸的设备中的应用以及在线粒体中检测SO ₃ ^2-的潜力

设计并合成了一种新型的水溶性荧光探针（L），该探针可以证明按比例检测100％水性介质和活细胞中的SO ₂衍生物（SO ₃ ^2-）。可以通过肉眼看到检测过程，因为L的橙红色荧光在与SO ₃ ^2-相互作用后变成了强烈的蓝色荧光。L显示了几个有益的属性，例如在完全水性介质中的检测，极快的响应时间以及高选择性和灵敏度。比率量度感测是由于SO的选择性亲核加成反应₃ ^2-与大号。该探针还用于开发低成本的微流体传感器设备（μPAD）。该探针具有生物相容性，并在活HeLa细胞中捕获了检测线粒体SO ₃ ^2-的潜力。