The nanoparticles comprising of iron core and carbon shell were decorated with ferrocene derivatives: ferrocenecarboxaldehyde (Fc-1) and ferrocenecarboxaldehyde oxime (Fc-2). A microdrop of suspension of the nanoconjugate was placed on a glassy-carbon electrode to prepare the recognition/sensing layer. Drying and purification of the sensing layer resulted in a well-defined and stable square-wave voltammogram of the ferrocene moiety. The height of the voltammetric peak increased in the presence of ceruloplasmin. That increase was linearly dependent on the logarithmic concentration of ceruloplasmin in blood. The applied external magnetic field was a factor which yielded better sensitivity and repeatability of the sensor response. The linearity of sensor response was found to be between 0.001 and 10 μg dL⁻¹ and 0.05–10 μg dL⁻¹ for both nanoconjugates: [email protected] and [email protected], in the presence and absence of the magnet, respectively. The obtained detection limit (LOD) for [email protected] was found to be 0.60 and 0.10 μg dL⁻¹ in the absence and presence of magnetic field, respectively, whilst for [email protected] was 0.4 and 0.07 μg dL⁻¹ in the absence and presence of a magnet, respectively. The proposed method is selective because the presence of common antioxidants in blood did not interfere significantly with the determination of the concentration of ceruloplasmin.

用二茂铁衍生物：二茂铁甲醛（Fc-1）和二茂铁甲醛肟（Fc-2）修饰由铁核和碳壳组成的纳米颗粒。将微滴的纳米共轭物悬浮液置于玻璃碳电极上以制备识别/传感层。感测层的干燥和纯化导致二茂铁部分的定义明确且稳定的方波伏安图。在存在铜蓝蛋白的情况下，伏安峰的高度增加。这种增加与血液中铜蓝蛋白的对数浓度线性相关。施加的外部磁场是产生更好的传感器响应灵敏度和可重复性的因素。发现传感器响应的线性在0.001和10μgdL ^-1之间和两种纳米共轭物的0.05-10μgdL ^-1：[存在磁铁的情况下]和[受到磁铁的情况下]，分别在有磁铁和无磁铁的情况下。发现在不存在和存在磁场的情况下，对[受电子邮件保护]的检测限（LOD）分别为0.60和0.10μgdL ^-1，而对受[电子邮件保护]的检测限分别为0.4和0.07μgdL ^-1。磁铁的不存在和存在。提议的方法是选择性的，因为血液中常见的抗氧化剂的存在不会显着干扰铜蓝蛋白浓度的测定。