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The application of CO 2 -sensitive AIEgen in studying the synergistic effect of stromal cells and tumor cells in a heterocellular system
Analytica Chimica Acta ( IF 6.2 ) Pub Date : 2018-02-01 , DOI: 10.1016/j.aca.2017.11.046
Didi Chen , Huan Wang , Pai Liu , Linlin Song , Jianbing Shi , Bin Tong , Yuping Dong

Reciprocal signaling between stromal and tumor cells was believed to contribute to tumor cell proliferation and apoptosis in heterocellular systems. Herein we used the CO2-sensitive AIEgen as bioprobe to study the synergistic effect of stromal cells and tumor cells in a heterocellular system. The experimental results demonstrated that metabolic rates of living tumor cells in the co-culture system were still faster than that of stromal cell through the detection of CO2 generation rate in living cell. All rates were, however, slower than that in homocellular system. It indicated that tumor cells would induce neighboring stromal cells to establish a common protection mechanism against foreign material invasion, which enhanced the cell activity and drug resistance. In addition, tumor cells in solid carcinoma exhibited delayed growth but less apoptosis in co-culture systems. Taken these results together, a bidirectional signaling pathway theory was proposed between tumor and stromal cells in co-culture system and could play a different and important role in anticancer drug molecules designs.

中文翻译:

CO 2 敏感性AIEgen在异细胞系统中基质细胞与肿瘤细胞协同效应研究中的应用

间质细胞和肿瘤细胞之间的相互信号被认为有助于异细胞系统中的肿瘤细胞增殖和凋亡。在此,我们使用 CO2 敏感的 AIEgen 作为生物探针来研究异细胞系统中基质细胞和肿瘤细胞的协同作用。实验结果表明,通过检测活细胞中的CO2生成率,共培养系统中活肿瘤细胞的代谢率仍然比基质细胞快。然而,所有速率都比同细胞系统慢。这表明肿瘤细胞会诱导邻近的基质细胞建立共同的保护机制,防止外来物质入侵,从而增强细胞活性和耐药性。此外,实体癌中的肿瘤细胞在共培养系统中表现出生长延迟但凋亡较少。综合这些结果,提出了共培养系统中肿瘤和基质细胞之间的双向信号通路理论,可以在抗癌药物分子设计中发挥不同的重要作用。
更新日期:2018-02-01
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