Microbial asparaginase is an essential component of chemotherapy for the treatment of childhood acute lymphoblastic leukemia (cALL). Silent hypersensitivity reactions to this microbial enzyme need to be monitored accurately during treatment to avoid adverse effects of the drug and its silent inactivation. Here, we present a dual-response anti-asparaginase sensor that combines indirect SPR and fluorescence on a single chip to perform ELISA-type immunosensing, and correlate measurements with classical ELISA. Analysis of serum samples from children undergoing cALL therapy revealed a clear correlation between single-chip indirect SPR/fluorescence immunosensing and ELISA used in clinical settings (R² > 0.9). We also report that the portable SPR/fluorescence system had a better sensitivity than classical ELISA to detect antibodies in clinical samples with low antigenicity. This work demonstrates the reliability of dual sensing for monitoring clinically relevant antibody titers in clinical serum samples.

中文翻译：

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使用单芯片间接等离子和荧光免疫传感技术跟踪白血病治疗过程中对天冬酰胺酶的沉默超敏反应

微生物天冬酰胺酶是用于治疗儿童急性淋巴细胞白血病（cALL）的化学疗法的重要组成部分。在治疗过程中需要准确监测对该微生物酶的沉默超敏反应，以避免药物的副作用及其沉默失活。在这里，我们提出了一种双反应抗天冬酰胺酶传感器，该传感器在单个芯片上结合了间接SPR和荧光以执行ELISA型免疫传感，并将测量值与经典ELISA相关联。对接受cALL治疗的儿童的血清样本进行分析后发现，单芯片间接SPR /荧光免疫传感与临床设置中使用的ELISA之间存在明显的相关性（R ²> 0.9）。我们还报告说，便携式SPR /荧光系统具有比传统ELISA更好的灵敏度，可检测具有低抗原性的临床样品中的抗体。这项工作证明了双重传感技术在监测临床血清样品中临床相关抗体滴度方面的可靠性。