A rapid and specific, hly-based, loop-mediated isothermal amplification (LAMP) was applied for the detection of Listeria monocytogenes in food and food products, using a real-time turbidimeter platform (LAMP-turbidity). The principle behind this method relies on an increase in a DNA yield, which correlates with the production of magnesium pyrophosphate, and the results can be determined via an amplification curve within 1 h. The specificity test revealed that L. monocytogenes (DMST 17303) was observed from 34.1 to 38.3 min, while thirty strains of non-L. monocytogenes demonstrated no cross-reactions. The limits of detection for purified genomic DNA and pure culture were 800 pg μL⁻¹ and 2.82 × 10³ CFU mL⁻¹, respectively. Investigation on 200 raw chicken meat samples indicated that the specificity, sensitivity, and accuracy of LAMP-turbidity were 100%, 62.75%, and 90.50%, respectively. These data suggest that an hly-based, real-time, quantitative LAMP-turbidity assay can be an applicable tool for the epidemiological screening of L. monocytogenes in food and food products.

中文翻译：

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使用实时环介导的等温扩增浊度测定法一步一步快速筛选食品样品中的李斯特菌

使用实时浊度仪平台（LAMP-浊度），将快速，特异性，基于hly的环介导的等温扩增（LAMP）应用于食品和食品中单核细胞增生李斯特菌的检测。该方法的原理依赖于DNA产量的增加，这与焦磷酸镁的产生有关，并且可以通过1 h内的扩增曲线确定结果。特异性测试表明，在34.1至38.3分钟内观察到了单核细胞增生李斯特氏菌（DMST 17303），而三十种非单核细胞增生李斯特氏菌菌株均未显示出交叉反应。纯化的基因组DNA和纯培养物的检出限为800 pgμL ^-1和2.82×10 ³ CFU mL ^-1。对200个生鸡肉样品的调查表明，LAMP浊度的特异性，敏感性和准确性分别为100％，62.75％和90.50％。这些数据表明，基于hly的实时定量LAMP浊度测定法可以作为适用于食品和食品中单核细胞增生李斯特菌流行病学筛查的工具。