The trace detection of toxic compounds in complex matrices is a major concern, in particular when it comes to mycotoxins in food. We developed a highly sensitive and specific SERS sensor for the detection of ochratoxin A using a simple rough gold film as a substrate. When adding the analyte, we observed spectral variations related to the interaction of the analyte with the specific aptamer used as a bioreceptor. Using a partial least squares regression method, our sensor is able to detect concentrations down to the picomolar range, which is much lower than the minimum legal concentration allowed in food products. Moreover, we demonstrate the accurate detection of the analyte in a wide concentration range from the picomolar up to the micromolar level. The detection was validated with negative detection tests using deoxynivalenol and bovine serum albumin.

中文翻译：

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表面增强拉曼散射传感器，用于高灵敏度和选择性检测曲霉毒素A

痕量检测复杂基质中的有毒化合物是一个主要问题，尤其是涉及食物中的霉菌毒素时。我们开发了一种高灵敏度和特异性的SERS传感器，使用简单的粗糙金膜作为底物来检测曲霉毒素A。添加分析物时，我们观察到与分析物与用作生物受体的特定适体相互作用有关的光谱变化。使用偏最小二乘回归方法，我们的传感器能​​够检测到皮摩尔级的浓度，该浓度远低于食品中允许的最低法定浓度。此外，我们证明了在从皮摩尔到微摩尔水平的宽浓度范围内，分析物的准确检测。