We explored the use of irreversible electroporation (IRE) and high-frequency irreversible electroporation (H-FIRE) to induce cell death of tumor-initiating cells using a mouse ovarian surface epithelial (MOSE) cancer model. Tumor-initiating cells (TICs) can be successfully destroyed using pulsed electric field parameters common to irreversible electroporation protocols. Additionally, high-frequency pulses seem to induce cell death of TICs at significantly lower electric fields suggesting H-FIRE can be used to selectively target TICs and malignant late-stage cells while sparing the non-malignant cells in the surrounding tissue. We evaluate the relationship between threshold for cell death from H-FIRE pulses and the capacitance of cells as well as other properties that may play a role on the differences in the response to conventional IRE versus H-FIRE treatment protocols.

中文翻译：

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高频不可逆电穿孔靶向卵巢癌中的弹性肿瘤起始细胞

我们探索了使用不可逆电穿孔（IRE）和高频不可逆电穿孔（H-FIRE）使用小鼠卵巢表面上皮癌（MOSE）癌症模型诱导肿瘤起始细胞的细胞死亡。使用不可逆电穿孔方案通用的脉冲电场参数可以成功破坏肿瘤起始细胞（TICs）。另外，高频脉冲似乎在较低的电场下诱导TICs的细胞死亡，这表明H-FIRE可用于选择性靶向TICs和恶性晚期细胞，同时保留周围组织中的非恶性细胞。我们评估了H-FIRE脉冲导致的细胞死亡阈值与细胞电容之间的关系，以及可能对常规IRE反应的差异发挥作用的其他特性与H-FIRE处理方案进行比较。