The risk of developing hypersensitivity to alternative antibiotics is a concern for penicillin hypersensitive patients and healthcare providers. Herein we use piperacillin hypersensitivity as a model to explore the reactivity of drug-specific IgG against alternative β-lactam protein adducts. Mass spectrometry was used to show the drugs (amoxicillin, flucloxacillin, benzyl penicillin, aztreonam, and piperacillin) bind to similar lysine residues on the protein carrier bovine serum albumin. However, the hapten-specific IgG antibodies found in piperacillin hypersensitive patient plasma did not bind to other β-lactam protein conjugates. These data outline the fine specificity of piperacillin-specific IgG antibodies that circulate in patients with hypersensitivity.

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评估抗哌拉西林IgG与结构相关药物蛋白加合物的结合

对青霉素超敏患者和医疗保健提供者来说，对替代抗生素产生超敏性的风险是关注的问题。在本文中，我们使用哌拉西林超敏反应作为模型，探讨药物特异性IgG对替代性β-内酰胺蛋白加合物的反应性。质谱用于显示药物（阿莫西林，氟氯西林，苄青霉素，氨曲南和哌拉西林）与蛋白质载体牛血清白蛋白上的相似赖氨酸残基结合。但是，在哌拉西林过敏患者血浆中发现的半抗原特异性IgG抗体不与其他β-内酰胺蛋白结合物结合。这些数据概述了在超敏反应患者中循环的哌拉西林特异性IgG抗体的优良特异性。