In this research, a hydrophilic polymeric monolith containing choline phosphate (CP) was fabricated through the thermally initiated free-radical polymerization of 2-{2-(methacryloyloxy) ethyldimethylammonium}ethyl n-butyl phosphate (MBP) with ethylene dimethacrylate (EDMA) using isopropanol and tetrahydrofuran as the porogenic system. Excellent mechanical strength, permeability, stability and reproducibility were obtained on the optimized poly(MBP-co-EDMA) monolith. Column efficiency as high as 107,500 plates/m was achieved for the analysis of thiourea at a flow velocity of 0.2 mm/s on the monolith. Both hydrophilic and electrostatic interactions were observed for the retention of charged analytes on the poly(MBP-co-EDMA) monolith. It is worth noting that the resulting monolith exhibits higher selectivity and efficiency than the classical 2-(methacryloyloxy)ethyl phosphorylcholine functionalized polymeric monolith for the chromatographic separation of polar analytes. Also, the novel monolithic column was successfully applied to enrich N-glycopeptides from tryptic digest of human IgG. In a word, the versatile MBP functionalized polymeric monolith not only opens up interesting possibilities for reverse zwitterionic CP derivative-based polymeric materials in separation science, but also represents a promising hydrophilic interaction chromatography stationary phase.

中文翻译：

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用于分离科学应用的含有磷酸胆碱的亲水聚合物整料

在本研究中，通过 2-{2-(甲基丙烯酰氧基) 乙基二甲基铵}乙基正丁基磷酸酯 (MBP) 与二甲基丙烯酸乙烯酯 (EDMA) 的热引发自由基聚合制备了含有磷酸胆碱 (CP) 的亲水聚合物整料。异丙醇和四氢呋喃作为致孔系统。优化的聚 (MBP-co-EDMA) 整料获得了优异的机械强度、渗透性、稳定性和重现性。柱效高达 107,500 塔板/米，用于以 0.2 毫米/秒的流速分析整体块上的硫脲。对于带电分析物在聚 (MBP-co-EDMA) 整料上的保留，观察到亲水和静电相互作用。值得注意的是，对于极性分析物的色谱分离，所得整体块比经典的 2-（甲基丙烯酰氧基）乙基磷酰胆碱官能化聚合物整体块具有更高的选择性和效率。此外，新型整体柱已成功应用于从人 IgG 的胰蛋白酶消化物中富集 N-糖肽。总之，多功能 MBP 功能化聚合物整料不仅为分离科学中基于反向两性离子 CP 衍生物的聚合物材料开辟了有趣的可能性，而且代表了一种有前途的亲水相互作用色谱固定相。新型整体柱已成功应用于从人 IgG 的胰蛋白酶消化物中富集 N-糖肽。总之，多功能 MBP 功能化聚合物整料不仅为分离科学中基于反向两性离子 CP 衍生物的聚合物材料开辟了有趣的可能性，而且代表了一种有前途的亲水相互作用色谱固定相。新型整体柱已成功应用于从人 IgG 的胰蛋白酶消化物中富集 N-糖肽。总之，多功能 MBP 功能化聚合物整料不仅为分离科学中基于反向两性离子 CP 衍生物的聚合物材料开辟了有趣的可能性，而且代表了一种有前途的亲水相互作用色谱固定相。