In this study, the pesticide sensor by graphene quantum dots (GQDs) and active enzyme, GQDs/enzyme platform, has been developed for monitoring the organophosphate pesticides. In this concept, H₂O₂ generated from the active enzymatic reaction of acetylcholinesterase (AChE) and choline oxidase (CHOx) enables to react with GQDs resulting in a “turn-off” photoluminescence of GQDs. A “turn-on” photoluminescence of GQDs at 467 nm was recovered in the presence of organophosphate. Definitely, the photoluminescence changes of GQDs/AChE/CHOx biosensor reasonably correspond to the amount of pesticide. The detection limit of GQDs/AChE/CHOx biosensor towards dichlorvos was 0.172 ppm (0.778 μM). In this approach, this biosensor offers the promising determination of the organophosphate pesticides and a benefit for easy checking of organophosphate pesticides in food, water and environment with low cost, easy-to-prepare and less toxic to environment.

在这项研究中，已开发出由石墨烯量子点（GQD）和活性酶GQD /酶平台组成的农药传感器，用于监测有机磷酸盐农药。在这个概念中，H ₂ O ₂乙酰胆碱酯酶（AChE）和胆碱氧化酶（CHOx）的主动酶促反应产生的碳能够与GQD反应，从而导致GQD的“关闭”光致发光。在存在有机磷酸酯的情况下，在467 nm处回收了GQD的“开启”光致发光。可以肯定的是，GQDs / AChE / CHOx生物传感器的光致发光变化与农药的含量合理地对应。GQDs / AChE / CHOx生物传感器对敌敌畏的检出限为0.172 ppm（0.778μM）。通过这种方法，该生物传感器提供了有前途的有机磷农药测定方法，并且具有易于检查食品，水和环境中的有机磷农药的优点，且成本低，易于制备且对环境的毒性较小。