A novel ratiometric fluorescent probe, ACC-Cl, was developed by virtue of a unique H₂S_n-mediated coumarindithiolone formation under mild condition. The probe ACC-Cl exhibited excellent sensitivity and selectivity toward H₂S_n over other RSS such as biothiols and H₂S. More importantly, titration of H₂S_n to the solution of ACC-Cl under the existence of 1 mM GSH, the obvious ratiometric fluorescence response still can be observed. Significantly, preliminary biological experiments indicated its potential for sensing H₂S_n in living cells.

中文翻译：

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合理开发用于多硫化氢的高选择性比例荧光探针

通过在温和条件下独特的H ₂ S _n介导的香豆素二硫酮形成，开发了一种新型的比例荧光探针ACC-Cl 。相对于其他RSS（例如生物硫醇和H ₂ S），探针ACC-Cl对H ₂ S _n表现出出色的灵敏度和选择性。更重要的是，在1 mM GSH存在下，将H ₂ S _n滴定到ACC-Cl溶液中，仍然可以观察到明显的比例荧光响应。重要的是，初步的生物学实验表明它具有检测活细胞中H ₂ S _n的潜力。