Herein, we report a new rhodamine-based multianalyte (Cu²⁺ and Hg²⁺) fluorescent sensor RH-FP. The synthesized probe acts as a dual chemosensor for recognition of Cu²⁺ and Hg²⁺ in acetonitrile-water (4:1 v/v, pH 7.2 with 10 mM, HEPES) in the presence of other miscellaneous metal cations i.e. Ag⁺, Al³⁺, Ba²⁺, Ca²⁺, Cd²⁺, Fe³⁺, Fe²⁺, K⁺, Li⁺, Mg²⁺, Mn²⁺, Cu⁺², Pb²⁺, Ni²⁺, Na⁺ and Zn²⁺. A highly selective and sensitive colorimetric (colorless to pink) response accompanied by a significant increase in absorption and emission maxima (250-folds) was observed by the interaction of RH-FP and Cu²⁺ or Hg²⁺. This makes RH-FP a suitable “bare-eye” detector. The Job’s plot and ESI–MS confirm 1:1 binding stoichiometry between sensor RH-FP and metal cations. The calculated binding constant for the complexes (RH-FP-Cu²⁺ and RH-FP-Hg²⁺) are 3.37 × 10⁵ mol⁻¹ and 7.6 × 10⁵ mol⁻¹, with a detection limit of 3.9 nm and 2.36 nm respectively. The binding mechanism was confirmed by Fourier transform infra-red spectroscopy (FT-IR) ¹H, ¹³C NMR and density functional theory (DFT) calculations. Moreover, the fluorescent sensor RH-FP displayed potential in-field applications as detection kit and in fluorescent imaging of biological systems to detect Cu²⁺ or Hg²⁺.

在此，我们报告了一种新型基于若丹明的多分析物（Cu ²⁺和Hg ²⁺）荧光传感器RH-FP。将合成的探针用作用于识别的Cu的双重化学传感器²⁺和Hg ²⁺在乙腈-水（4:1 V / V，PH值7.2的10mM，HEPES）中其他杂项金属阳离子的存在，即银⁺， Al ³⁺，Ba 2 ⁺，Ca 2 ⁺，Cd 2 ⁺，Fe ³⁺，Fe 2 ⁺，K ⁺，Li ⁺，Mg 2 ⁺，Mn 2 ⁺，Cu ⁺²，Pb ²⁺，Ni 2 ⁺，Na ⁺和Zn ²⁺。通过RH-FP与Cu ²⁺或Hg ²⁺的相互作用，观察到了高度选择性和灵敏的比色反应（无色至粉红色），同时吸收和发射最大值显着增加（250倍）。这使得RH-FP成为合适的“裸眼”检测器。乔布的图和ESI-MS证实了传感器RH-FP和金属阳离子之间的化学计量比为1：1 。计算出的配合物（RH-FP-Cu ²⁺和RH-FP-Hg ²⁺）的结合常数为3.37×10 ⁵  mol ^-1和7.6×10 ⁵ mol ^-1，检测极限分别为3.9 nm和2.36 nm。通过傅立叶变换红外光谱（FT-IR）¹ H，¹³ C NMR和密度泛函理论（DFT）计算确认了结合机理。此外，荧光传感器RH-FP在检测试剂盒中以及在生物系统的荧光成像中可检测Cu ²⁺或Hg ^2+的应用中显示出潜在的现场应用。