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Strategies for MSC expansion and MSC-based microtissue for bone regeneration.
Biomaterials ( IF 14.0 ) Pub Date : 2017-11-21 , DOI: 10.1016/j.biomaterials.2017.11.023
Varitsara Bunpetch 1 , Zhi-Yong Zhang 2 , Xiaoan Zhang 1 , Shan Han 1 , Pan Zongyou 1 , Haoyu Wu 1 , Ouyang Hong-Wei 3
Affiliation  

Mesenchymal stem cells (MSCs) have gained increasing attention as a potential approach for the treatment of bone injuries due to their multi-lineage differentiation potential and also their ability to recognize and home to damaged tissue sites, secreting bioactive factors that can modulate the immune system and enhance tissue repair. However, a wide gap between the number of MSCs obtainable from the donor site and the number required for implantation, as well as the lack of understanding of MSC functions under different in vitro and in vivo microenvironment, hinders the progression of MSCs toward clinical settings. The clinical translation of MSCs pre-requisites a scalable expansion process for the biomanufacturing of therapeutically qualified cells. This review briefly introduces the features of implanted MSCs to determine the best strategies to optimize their regenerative capacity, as well as the current MSC implantation for bone diseases. Current achievements for expansion of MSCs using various culturing methods, bioreactor technologies, biomaterial platforms, as well as microtissue-based expansion strategies are also discussed, providing new insights into future large-scale MSC expansion and clinical applications.

中文翻译:

MSC扩张和基于MSC的骨再生微组织的策略。

间充质干细胞(MSCs)由于具有多系分化潜能,并且具有识别和归巢受损组织部位的能力,并能分泌可调节免疫系统的生物活性因子,因此作为治疗骨损伤的潜在方法受到越来越多的关注。并增强组织修复。但是,可从供体部位获得的MSC数量与植入所需的数量之间存在很大差距,并且缺乏对不同体外和体内微环境下MSC功能的了解,这阻碍了MSC向临床环境的发展。MSC的临床翻译为治疗合格细胞的生物制造提供了可扩展的扩展过程。这篇综述简要介绍了植入的MSC的特性,以确定最佳的策略以优化其再生能力,以及当前针对骨骼疾病的MSC植入。还讨论了使用各种培养方法,生物反应器技术,生物材料平台以及基于微组织的扩增策略扩增MSC的最新成就,从而为未来的大规模MSC扩增和临床应用提供了新见识。
更新日期:2018-11-29
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