Safe multifunctional nanoplatforms that have multiple therapeutic functions integrated with imaging capabilities are highly desired for biomedical applications. In this paper, targeted chemo–photothermal synergistic therapy and photoacoustic/computed tomography imaging of tumors were achieved by one novel multifunctional nanoprobe (GMS/DOX@SLB-FA); it was composed of a gold nanostar core and a doxorubicin (DOX)-loaded mesoporous silica shell (GMS), which was coated with a folic acid (FA)-modified thermosensitively supported lipid bilayer (SLB-FA) as a gatekeeper. The multifunctional probe had perfect dispersion and stability; 2.1 nm mesoporous pores and 208 nm hydration particle sizes were obtained. In vitro studies indicated that the drug-loaded probe had excellent ability to control the release of DOX, with 71.98 ± 2.52% cumulative release after laser irradiation, which was significantly higher than that of unirradiated control group. A survival rate of 72.75 ± 4.37% of HeLa cells at 57.75 μg/mL probe also demonstrated the low cytotoxicity of the targeted probe. Both in vitro and in vivo results showed that the probe could achieve targeted photoacoustic imaging of tumors because of the fact that the FA-modified probe could specifically recognize the overexpressed FA receptors on tumor cells; meanwhile, the probe could also achieve the chemo–photothermal synergistic therapy of tumors through controlling the drug release from mesoporous channels by a near-infrared laser. Therefore, the probe had great potential in the early diagnosis and treatment of cancer.

中文翻译：

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基于金纳米星，中孔二氧化硅和热敏脂质体复合纳米探针的体内计算机断层扫描/光声成像和近红外触发的化学-光热联合疗法。

具有多种治疗功能和成像功能的安全多功能纳米平台是生物医学应用的迫切需求。本文通过一种新型的多功能纳米探针（GMS / DOX @ SLB-FA）实现了针对性的化学-光热协同治疗和肿瘤的光声/计算机断层摄影成像。它由金纳米星核和阿霉素（DOX）负载的介孔二氧化硅壳（GMS）组成，该壳涂有叶酸（FA）修饰的热敏支撑脂质双层（SLB-FA）作为看门人。多功能探针具有良好的分散性和稳定性；获得了2.1 nm的中孔和208 nm的水合粒径。体外研究表明，载有药物的探针具有出色的控制DOX释放的能力，为71.98±2。激光辐照后的累积释放率为52％，显着高于未辐照对照组。在57.75μg/ mL探针下，HeLa细胞的存活率为72.75±4.37％，也证明了靶向探针的低细胞毒性。体外和体内结果均表明，该探针可以实现肿瘤的靶向光声成像，因为FA修饰的探针可以特异性识别肿瘤细胞上过表达的FA受体。同时，该探针还可以通过控制近红外激光从介孔通道释放药物来实现肿瘤的化学-光热协同治疗。因此，该探针在癌症的早期诊断和治疗中具有巨大的潜力。在57.75μg/ mL探针下，HeLa细胞的存活率为72.75±4.37％，也证明了靶向探针的低细胞毒性。体外和体内结果均表明，该探针可以实现肿瘤的靶向光声成像，因为FA修饰的探针可以特异性识别肿瘤细胞上过表达的FA受体。同时，该探针还可以通过控制近红外激光从介孔通道释放药物来实现肿瘤的化学-光热协同治疗。因此，该探针在癌症的早期诊断和治疗中具有巨大的潜力。在57.75μg/ mL探针下，HeLa细胞的存活率为72.75±4.37％，也证明了靶向探针的低细胞毒性。体外和体内结果均表明，该探针可以实现肿瘤的靶向光声成像，因为FA修饰的探针可以特异性识别肿瘤细胞上过表达的FA受体。同时，该探针还可以通过控制近红外激光从介孔通道释放药物来实现肿瘤的化学-光热协同治疗。因此，该探针在癌症的早期诊断和治疗中具有巨大的潜力。体外和体内结果均表明，该探针可以实现肿瘤的靶向光声成像，因为FA修饰的探针可以特异性识别肿瘤细胞上过表达的FA受体。同时，该探针还可以通过控制近红外激光从介孔通道释放药物来实现肿瘤的化学-光热协同治疗。因此，该探针在癌症的早期诊断和治疗中具有巨大的潜力。体外和体内结果均表明，该探针可以实现肿瘤的靶向光声成像，因为FA修饰的探针可以特异性识别肿瘤细胞上过表达的FA受体。同时，该探针还可以通过控制近红外激光从介孔通道释放药物来实现肿瘤的化学-光热协同治疗。因此，该探针在癌症的早期诊断和治疗中具有巨大的潜力。