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Role of solvent properties of water in crowding effects induced by macromolecular agents and osmolytes
Molecular BioSystems Pub Date : 2017-09-20 00:00:00 , DOI: 10.1039/c7mb00436b
L. A. Ferreira 1, 2, 3 , V. N. Uversky 3, 4, 5, 6, 7 , B. Y. Zaslavsky 1, 2, 3
Affiliation  

Solvent properties of water in aqueous solutions of polyethylene glycols of various molecular weights, L-proline, betaine, and a series of chlorides of varied concentrations are assayed using three solvatochromic dyes. The properties include solvent dipolarity/polarizability, hydrogen bond donor acidity, and hydrogen bond acceptor basicity. These properties are also evaluated in mixtures of two polymers, polymer and osmolyte, and two osmolytes. It is shown that linear combinations of solvent dipolarity/polarizability and hydrogen bond donor acidity assayed in individual solutions of crowders strongly correlate with the effects of the crowders on the stability of various proteins and nucleic acids reported in the literature. The solvent properties of water in aqueous mixtures of two macromolecular crowders, two osmolytes, or mixtures of an osmolyte and a macromolecular crowder vary differently for various solvent properties. The overall effects of the two components in the mixture on a given solvent property of water may be additive, reduced or enhanced depending on the particular composition of the mixture. It is hypothesized that changes in the solvent properties of water are related to changes in the water hydrogen-bonding structuring. It is suggested that the observed crowder-induced changes in the solvent properties of water should be taken into account in theoretical considerations of crowding effects in biological systems.

中文翻译:

水的溶剂性质在大分子试剂和渗透液诱导的拥挤效应中的作用

各种分子量L的聚乙二醇水溶液中水的溶剂性质-脯氨酸,甜菜碱和一系列不同浓度的氯化物使用三种溶剂变色染料进行测定。这些性质包括溶剂偶极/可极化性,氢键供体酸度和氢键受体碱性。在两种聚合物(聚合物和渗透压剂,以及两种渗透压剂)的混合物中也评估了这些性能。结果表明,在拥挤剂的单独溶液中测定的溶剂偶极/极化度和氢键供体酸度的线性组合与拥挤剂对文献中报道的各种蛋白质和核酸稳定性的影响密切相关。对于两种不同的溶剂特性,两种大分子拥挤剂,两种渗透压剂的混合物或渗透压剂与大分子拥挤剂的混合物中的水的溶剂性质会有所不同。混合物中两种组分对水的给定溶剂性能的总体影响可加,减或增强,这取决于混合物的特定组成。假设水的溶剂性质的变化与水氢键结构的变化有关。建议在理论上考虑生物系统中的拥挤效应时,应考虑观察到的拥挤引起的水的溶剂特性变化。
更新日期:2017-11-21
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