Electrochemiluminescence (ECL) has been engineered to perform various tasks in the area of immunoassays and molecular diagnosis. However, there is still substantial potential for developments of ECL assay with high efficiency to achieve trace analysis. Herein, we demonstrate a polymer-amplified ECL assay via construction of linear Ru(bpy)₃²⁺–polymer. This new polymer material compensates for the relatively low ECL intensity from single ECL luminophore and realizes a stable and controllable labeling process. The polymer-amplified ECL assay achieved a remarkable sensitivity of 100 amol. The wide-ranging applications of the polymer-amplified ECL assay for Hepatitis B virus, carcinoembryonic antigen, 16sRNA, and thrombin also demonstrate its superiority. Hence, the polymer-amplified ECL assay possesses the potential to create a new paradigm in amplified ECL assays that could provide outstanding performance for biomedical analysis.

中文翻译：

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线性Ru（bpy）_{3 2} ^+-聚合物作为可检测电化学标记信号放大比的生物标志物的通用探针

电化学发光（ECL）经过精心设计，可以执行免疫测定和分子诊断领域的各种任务。但是，仍然有很大的潜力开发高效的ECL分析以实现痕量分析。在这里，我们通过线性Ru（bpy）₃ ^2+的构建证明了聚合物放大的ECL分析-聚合物。这种新的聚合物材料补偿了单个ECL发光体产生的相对较低的ECL强度，并实现了稳定且可控的标记过程。聚合物扩增的ECL分析实现了100 amol的显着灵敏度。聚合物扩增的ECL测定法在乙型肝炎病毒，癌胚抗原，16sRNA和凝血酶的广泛应用也证明了其优越性。因此，聚合物扩增的ECL分析具有在扩增的ECL分析中创建新范式的潜力，该范式可为生物医学分析提供出色的性能。