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A multi-functional gold/iron-oxide nanoparticle-CNT hybrid nanomaterial as virus DNA sensing platform
Biosensors and Bioelectronics ( IF 12.6 ) Pub Date : 2017-11-21 , DOI: 10.1016/j.bios.2017.11.052
Jaewook Lee , Masahiro Morita , Kenshin Takemura , Enoch Y. Park

Nanoparticle (NP)-carbon nanomaterial hybrid structures have garnered significant attention due to their synergistic effects. Based on their interesting properties, hybrid nanomaterial-based sensing platforms have been investigated to achieve high sensitivity and selectivity detection. In our study, binary-NP-decorated carbon nanotubes (bNP-CNTs) were successfully synthesized through a simple two-step method and applied as a biosensing platform. Gold (Au)/iron-oxide magnetic NP-decorated CNTs (Au/MNP-CNT) were used for influenza and norovirus DNA sensing channels. To demonstrate biosensing, first, the Au/MNP-CNTs were magnetically aligned on a Pt-interdigitated electrode, and then, a thiol-group-functionalized probe DNA was attached to the Au NP surface on the bNP-CNT hybrid structure through thiol chemistry. DNA hybridization between the target influenza or norovirus DNA and probe DNA was measured to monitor an electrical conductivity change of the Au/MNP-CNTs. Various concentrations of target DNA from 1 pM to 10 nM were monitored, and the limits of detection for influenza virus and norovirus were calculated to be approximately 8.4 pM and 8.8 pM, respectively. The specificity was confirmed using different mismatched DNA sequences, showing high specificity. Therefore, this Au/MNP-CNT-based DNA sensing system exhibited excellent detection potential, and such hybrid materials could be universally applied as a highly sensitive and selective biosensing system.



中文翻译:

多功能金/铁氧化物纳米碳纳米管杂化纳米材料作为病毒DNA传感平台

纳米颗粒(NP)-碳纳米材料的杂化结构由于其协同作用而备受关注。基于其有趣的特性,已对基于混合纳米材料的传感平台进行了研究,以实现高灵敏度和选择性检测。在我们的研究中,通过简单的两步法成功地合成了装饰有二元NP的碳纳米管(bNP-CNT),并将其用作生物传感平台。金(Au)/氧化铁磁性NP修饰的CNT(Au / MNP-CNT)用于流感和诺如病毒DNA感测通道。为了证明生物传感,首先,将Au / MNP-CNT在Pt叉指电极上进行磁性排列,然后,通过硫醇化学将巯基官能化的探针DNA连接到bNP-CNT杂化结构上的Au NP表面。测量靶流感或诺如病毒DNA与探针DNA之间的DNA杂交,以监测Au / MNP-CNT的电导率变化。监测了从1 pM到10 nM的各种浓度的靶DNA,计算出的流感病毒和诺如病毒的检出限分别约为8.4 pM和8.8 pM。使用不同的错配DNA序列证实了特异性,显示出高特异性。因此,该基于Au / MNP-CNT的DNA感测系统表现出优异的检测潜力,并且这种杂化材料可以普遍地用作高灵敏度和选择性的生物感测系统。流感病毒和诺如病毒的检出限分别约为8.4 pM和8.8 pM。使用不同的错配DNA序列证实了特异性,显示出高特异性。因此,该基于Au / MNP-CNT的DNA感测系统表现出优异的检测潜力,并且这种杂化材料可以普遍地用作高灵敏度和选择性的生物感测系统。流感病毒和诺如病毒的检出限分别约为8.4 pM和8.8 pM。使用不同的错配DNA序列证实了特异性,显示出高特异性。因此,该基于Au / MNP-CNT的DNA感测系统表现出优异的检测潜力,并且这种杂化材料可以普遍地用作高灵敏度和选择性的生物感测系统。

更新日期:2017-11-21
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