Occurrence and application of oligonucleotide probes have promoted great progress in the biochemical analysis field due to their unique biological and chemical properties. In this work, a long-stem shaped multifunctional molecular beacon (LS-MMB) that is responsive to a cancer-related gene, p53, is well-prepared. By designing the probe with long-paired bases at its two ends and short-paired bases between the middle region and the 3′ end, the LS-MMB is intelligently endowed with the ability to recognize the target analyte, serve as the polymerization primer/template, and signal the hybridization event synchronously, which is distinctly advantageous over the traditional molecular beacons (MBs). Moreover, it is excitingly found that the LS-MMB can be employed to exert intramolecular and intermolecular interactions for strand displacement amplification (SDA) without the involvement of any assistant probes; this therapy results in a really easy and rapid sensing system that provides an extremely low background noise and high target output signal. In this case, an excellent sensitivity and specificity to detect target gene down to picomolar level and resolution to even one nucleotide variation are achieved, respectively. In addition, the application potential for real genomic DNA analysis is realized. We envision that the probe of LS-MMB can act as a universal platform for biosensing and biomedical research.

中文翻译：

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长阀杆成形为用于高灵敏度的核酸确定的多官能分子信标通过分子内和分子间相互作用的基于链置换扩增

寡核苷酸探针的出现和应用由于其独特的生化特性而在生化分析领域取得了巨大的进步。在这项工作中，准备了对癌症相关基因p53有反应的长柄形多功能分子信标（LS-MMB）。通过设计在两端具有长对碱基，在中间区域和3'末端之间具有短对碱基的探针，LS-MMB具有智能识别能力，可识别目标分析物，用作聚合引物/模板，并同步发出杂交事件信号，这明显优于传统的分子信标（MBs）。而且，令人兴奋的发现，LS-MMB可用于分子内和分子间相互作用，以进行链置换扩增（SDA），而无需任何辅助探针的参与。这种疗法产生了一个真正简单且快速的传感系统，该系统提供了极低的背景噪声和高目标输出信号。在这种情况下，分别获得了极低的灵敏度和特异性，可检测到低至皮摩尔水平的靶基因，甚至可以分辨出一个核苷酸变异。另外，实现了实际基因组DNA分析的应用潜力。我们设想LS-MMB的探针可以作为生物传感和生物医学研究的通用平台。这种疗法产生了一个真正简单且快速的传感系统，该系统提供了极低的背景噪声和高目标输出信号。在这种情况下，分别获得了极低的灵敏度和特异性，可检测到低至皮摩尔水平的靶基因，甚至可以分辨出一个核苷酸变异。另外，实现了实际基因组DNA分析的应用潜力。我们设想LS-MMB的探针可以作为生物传感和生物医学研究的通用平台。这种疗法产生了一个真正简单且快速的传感系统，该系统提供了极低的背景噪声和高目标输出信号。在这种情况下，分别获得了极低的灵敏度和特异性，可检测到低至皮摩尔水平的靶基因，甚至可以分辨出一个核苷酸变异。另外，实现了实际基因组DNA分析的应用潜力。我们设想LS-MMB的探针可以作为生物传感和生物医学研究的通用平台。