Sulfur dioxide (SO₂) is used as a universal additive to wine due to its excellent antioxidative, antimicrobic, and anti-brown characteristics and also because it is the product of natural fermentation of grapes. The concentration of SO₂ in wine is strictly regulated since SO₂ at high concentrations can damage tissues, organs, and various systems of human beings. In this study, an atomic fluorescence spectrometer (AFS) was used for the determination of SO₂ by measuring its molecular fluorescence signal excited by a zinc hollow cathode lamp (Zn HCL, 213.8 nm) under simple and mild conditions without igniting the atomizer. The instrumental settings were first optimized by detecting gaseous SO₂ prepared in advance. Under the optimal conditions, the instrumental limit of detection (LOD) for SO₂ was found to be 0.17 μg, and the relative standard deviation (RSD) of 200 μg SO₂ was 1.3%. The method was developed for determining the total SO₂ in wine samples extracted by sodium hydroxide and generated in the form using sulfuric acid. The LOD of 0.79 μg mL⁻¹ for this method was achieved for the total SO₂ analysis of wine samples with a 5.2% RSD at a concentration level of 30 μg mL⁻¹. The advantages of this method are simplicity, high throughput, less interference, relatively low cost, and less chemical consumption.

中文翻译：

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在线化学蒸汽产生，使用原子荧光光谱仪测定葡萄酒样品中的总二氧化硫

二氧化硫（SO ₂）由于具有出色的抗氧化，抗微生物和抗棕色特性，并且是葡萄天然发酵的产物，因此被用作葡萄酒的通用添加剂。葡萄酒中SO ₂的浓度受到严格控制，因为高浓度的SO ₂会损害组织，器官和人类的各种系统。在这项研究中，使用原子荧光光谱仪（AFS）通过在简单和温和的条件下测量锌空心阴极灯（Zn HCL，213.8 nm）激发的分子荧光信号而无需点燃雾化器来测定SO ₂。首先通过检测气态SO _2来优化仪器设置事先准备。在最佳条件下，检测（LOD）为SO的仪器极限₂被发现是0.17微克，相对标准偏差（RSD）200微克的SO ₂为1.3％。该方法用于测定由氢氧化钠提取并以硫酸形式生成的葡萄酒样品中的总SO ₂。对于浓度为30μgmL ^-1的5.2％RSD的葡萄酒样品，总SO ₂分析实现了该方法的LOD为0.79μgmL ^-1。该方法的优点是简单，高通量，较少的干扰，相对较低的成本和较少的化学消耗。