A concept of using thin-layer chromatography to multicomponent sample preparation for quantitative determination of solutes followed by instrumental technique is presented. Thin-layer chromatography (TLC) is used to separate chosen substances and their internal standard from other components (matrix) and to form a single spot/zone containing them at the solvent front position. The location of the analytes and internal standard in the solvent front zone allows their easy extraction followed by quantitation by HPLC.

中文翻译：

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薄层色谱法作为多组分样品制备方法的溶剂前位提取方法，用于通过仪器技术进行定量分析

提出了使用薄层色谱法对多组分样品进行制备以定量测定溶质的概念，然后介绍了仪器技术。薄层色谱（TLC）用于将选定的物质及其内标与其他组分（基质）分开，并在溶剂的前端位置形成一个包含它们的单一斑点/区域。分析物和内标物在溶剂前沿区域中的位置使其易于提取，然后通过HPLC进行定量。