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Optimization of enzymatic production of prebiotic galacto/galacto(arabino)-oligosaccharides and oligomers from potato rhamnogalacturonan I
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2017-11-02 , DOI: 10.1016/j.carbpol.2017.11.011
Nastaran Khodaei , Salwa Karboune

A bi-enzymatic system using two multi-enzymatic preparations (Depol 670 L and Gamanase 1.5 L) was investigated for the production of prebiotic galacto/galacto(arabino)-oligosaccharides and oligomers with well-defined degree of polymerisation (DP) from potato galactan-rich rhamnogalacturonan I. Depending on the reaction condition, yields of low (DP of 2–6) and high-MW oligosaccharides (DP of 7–12) and oligomers (DP of 13–70) varied between 0.1–13.9, 0.0–37.5 and 0.0–75.7%, respectively. Substrate concentration and Depol 670 L/Gamanase 1.5 L ratio were identified as the most significant linear terms in oligosaccharide and oligomer yield models, respectively. Moreover, interaction between reaction time and substrate concentration had a significant effect on the yield of oligosaccharides, while interaction between reaction time and Depol 670 L/Gamanase 1.5 L ratio affected significantly the yield of oligomers. Higher yields of both oligosaccharides and oligomers were obtained when equal amount of Depol 670L and Gamanase 1.5 L was used in combination. The DP and the monosaccharide composition of the generated galacto/galacto(arabino)-oligosaccharides and oligomers were confirmed.



中文翻译:

马铃薯鼠李半乳糖醛酸聚糖酶I酶法生产益生元半乳/半乳(阿拉伯糖)-寡糖和低聚物的工艺优化

研究了一种使用两种多酶制剂(Depol 670  L和Gamanase 1.5  L)的双酶体系,用于从马铃薯半乳聚糖生产具有明确定义的聚合度(DP)的益生元半乳/半乳(阿拉伯)-寡糖和低聚物。富含鼠李糖半乳糖醛酸聚糖I.根据反应条件,低(DP为2–6)和高分子量低聚糖(DP为7–12)和低聚物(DP为13–70)的产量在0.1–13.9、0.0–2之间变化。分别为37.5和0.0–75.7%。底物浓度和Depol 670  L / Gamanase 1.5 在寡糖和低聚物产率模型中,L比分别被确定为最重要的线性项。此外,反应时间和底物浓度之间的相互作用对寡糖的产率有显着影响,而反应时间和Depol 670  L / Gamanase 1.5  L的比例之间的相互作用显着影响寡聚体的产率。当同时使用等量的Depol 670L和Gamanase 1.5  L时,寡糖和寡聚体的产率都较高。确认了生成的半乳/半乳(阿拉伯)-寡糖和寡聚物的DP和单糖组成。

更新日期:2017-12-06
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