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In vivo study of erysolin metabolic profile by ultra high performance liquid chromatography coupleded to Fourier transform ion cyclotron resonance mass spectrometry
Journal of Chromatography B ( IF 3 ) Pub Date : 2017-11-16 , DOI: 10.1016/j.jchromb.2017.11.019
Binglong Li , Hui Zhou , Guochun Yang , Fei Han , Yanting Li , Yongfeng Gao , Jinwei Gao , Feng Zhang , Lixin Sun

An ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was developed for the first time to study the in vivo metabolism of erysolin, a compound derived from cruciferous plants which has a definite effect of anti-tumor and anti-nerve injury. In this research, the chromatographic separation was performed on an ACQUITY UPLC® BEH C18 column (2.1 mm × 100 mm, 1.7 μm, Waters, USA) and eluted by a gradient program, the identification work was achieved on a Bruker ultra-high resolution spectrometer in positive ion mode. Plasma, urine, feces and bile samples were collected from rats to screen metabolites after an intragastric administration of erysolin at the dose of 100 mg/kg. As a result, the parent drug and a total of six phase II metabolites were detected and preliminarily identified by analyzing their MS and MS/MS spectrometry profiles. Our results indicated that erysolin mainly metabolized via the mercapturic acid metabolic pathway, erysolin first react with glutathione to form glutathione conjugate, followed by taking off the glutamic acid and glycine to form cysteine conjugate, then the N-acetylation reaction occurs, the product would be excreted out of the body at last. In conclusion, results obtained in our study may contribute to a better understanding of the metabolism process and characteristics of erysolin in vivo, and provide an important reference for future research.



中文翻译:

超高效液相色谱-傅里叶变换离子回旋共振质谱联用技术研究紫草素代谢特性

首次开发了一种结合傅里叶变换离子回旋共振质谱(UHPLC-FT-ICR-MS)方法的超高效液相色谱法,以研究紫杉醇的体内代谢,紫杉醇是十字花科植物中具有一定成分的化合物抗肿瘤和抗神经损伤作用。在这项研究中,上ACQUITY UPLC进行色谱分离®BEH C18色谱柱(2.1 mm×100 mm,1.7μm,美国沃特世),并通过梯度洗脱,使用正离子模式的布鲁克超高分辨率光谱仪完成了鉴定工作。从大鼠中收集血浆,尿液,粪便和胆汁样品后,以100 mg / kg的剂量在胃内注射异草素,以筛选代谢产物。结果,通过分析其母体和MS / MS光谱图谱,检测并初步鉴定了母体药物和总共6种II期代谢物。我们的研究结果表明,赤藓素主要通过巯基酸代谢途径代谢,赤藓素首先与谷胱甘肽反应形成谷胱甘肽共轭物,然后脱除谷氨酸和甘氨酸形成半胱氨酸共轭物,然后发生N-乙酰化反应,产品最终将被排出体外。总之,我们研究中获得的结果可能有助于更好地理解赤霉素的代谢过程和特征。体内,为今后的研究提供重要参考。

更新日期:2017-11-16
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