Genome editing using engineered nucleases (meganucleases, zinc finger nucleases, transcription activator-like effector nucleases) has created many recent breakthroughs. Prescreening for efficiency and specificity is a critical step prior to using any newly designed genome editing tool for experimental purposes. The current standard screening methods of evaluation are based on DNA sequencing or use mismatch-sensitive endonucleases. They can be time-consuming and costly or lack reproducibility. Here, we review and critically compare standard techniques with those more recently developed in terms of reliability, time, cost, and ease of use.

中文翻译：

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评价基因组编辑效果的技术比较

使用工程核酸酶（大范围核酸酶，锌指核酸酶，转录激活因子样效应子核酸酶）进行基因组编辑已创造了许多新突破。在使用任何新设计的基因组编辑工具进行实验之前，对效率和特异性进行预筛选是至关重要的一步。当前的评估标准筛选方法是基于DNA测序或使用错配敏感的核酸内切酶。它们可能既耗时又昂贵，或者缺乏可重复性。在这里，我们将就可靠性，时间，成本和易用性方面对标准技术与最近开发的标准技术进行审查并进行严格的比较。