Here, we report the developed cyclo olefin polymer (COP) microfluidic chip on a fabricated rotating heater stage that utilizes centrifugation-assisted thermal cycle in a ring-structured microchannel for polymerase chain reaction (PCR). The PCR solution could be driven by thermal convection and continuously exchanged high/low temperatures in a ring structured microchannel without the use of typical syringe pump. More importantly, the flow rate was controlled by the relative gravitational acceleration only. The platform enables amplification within 10 min at 5G and has a detection limit of 70.5 pg/channel DNA concentration (β-actin, 295 bp). The current rotating system is capable of testing four different samples in parallel. The microfluidic chip can be preloaded with the PCR premix solution for on-site utility, and, with all of the features integrated to the system, the test can be conducted without the need for specialized laboratory and trained laboratory staff. In addition, this innovative chemical reaction technique has the potential to be utilized in other micromixing applications.

中文翻译：

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离心控制的热对流及其在快速微流聚合酶链反应装置中的应用

在这里，我们报告了在制造的旋转加热器平台上开发的环烯烃聚合物（COP）微流控芯片，该平台利用离心辅助的热循环在环状结构微通道中进行聚合酶链反应（PCR）。PCR溶液可以通过热对流驱动，并且可以在不使用典型的注射泵的情况下在环形微通道中连续交换高/低温。更重要的是，流速仅由相对重力加速度控制。该平台可在10分钟内以5G扩增，并具有70.5 pg /通道DNA浓度（β-肌动蛋白，295 bp）的检测极限。当前的旋转系统能够并行测试四个不同的样本。可将微流体芯片预装PCR预混液以进行现场应用，并且，通过将所有功能集成到系统中，可以进行测试，而无需专门的实验室和训练有素的实验室人员。另外，这种创新的化学反应技术具有在其他微混合应用中使用的潜力。