Bimetallic gold/silver nanoclusters were synthesized in aqueous solution with lysozyme as a stabilizing and reducing agent. The prepared lysozyme-stabilized gold/silver nanoclusters (Lys-Au/Ag NCs) displayed highly near-infrared fluorescence and excellent stability under various pH values and ionic environments. We found that ascorbic acid (AA) significantly enhanced the fluorescence of Lys-Au/Ag NCs and L-ascorbyl-2-phosphate (PAA) had little effect on the fluorescence of Lys-Au/Ag NCs. Under acidic conditions, acid phosphatase (ACP) can catalyze the hydrolysis of PAA to generate AA molecules, enhancing the fluorescence of Lys-Au/Ag NCs. Therefore, we utilized the novel Lys-Au/Ag NCs as a turn-on fluorescent probe for the simple and convenient determination of ACP in the presence of PAA molecules.

中文翻译：

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溶菌酶稳定的双金属金/银纳米簇作为开启荧光探针，用于测定抗坏血酸和酸性磷酸酶†

在水溶液中以溶菌酶作为稳定剂和还原剂合成了双金属金/银纳米簇。制备的溶菌酶稳定的金/银纳米簇（Lys-Au / Ag NCs）在各种pH值和离子环境下均显示出高度近红外荧光和出色的稳定性。我们发现抗坏血酸（AA）显着增强了Lys-Au / Ag NCs的荧光，而L-抗坏血酸-2-磷酸酯（PAA）对Lys-Au / Ag NCs的荧光影响很小。在酸性条件下，酸性磷酸酶（ACP）可以催化PAA水解以生成AA分子，从而增强Ly​​s-Au / Ag NCs的荧光。因此，我们利用新颖的Lys-Au / Ag NCs作为开启荧光探针，在PAA分子存在下简单，方便地测定ACP。