Fluorophores for efficient long-term bioimaging are of great importance to fully understand the cellular and molecular processes of disease. In this study, a bright and photostable cationic fluorophore (PPB) was successfully developed as a long-term tracer. The PPB displayed advanced properties such as high fluorescence efficiency, large Stokes shift, low cytotoxicity, and good biocompatibility. Moreover, the PPB exhibited comparable photostability to the commercial cell tracker Qtracker 585. More importantly, the PPB can trace HeLa cells as long as 16 passages in vitro and monitor tumor growth for 27 days in vivo. These remarkable features endow the PPB as an ideal fluorescent probe for long-term bioimaging applications.

中文翻译：

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用于长期生物成像的光稳定阳离子荧光团

高效长期生物成像的荧光团对于充分了解疾病的细胞和分子过程非常重要。在这项研究中，成功开发出一种明亮且光稳定的阳离子荧光团（PPB）作为长期示踪剂。的PPB显示先进性能，例如高的荧光效率，大的斯托克斯频移，低细胞毒性和良好的生物相容性。此外，PPB表现出与商用细胞追踪器Qtracker 585相当的光稳定性。更重要的是，PPB可以在体外长达16代时追踪HeLa细胞，并在体内监测肿瘤生长27天。这些卓越的功能赋予了PPB 作为长期生物成像应用的理想荧光探针。