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Genome sequence of the progenitor of the wheat D genome Aegilops tauschii
Nature ( IF 64.8 ) Pub Date : 2017-11-01 , DOI: 10.1038/nature24486
Ming-Cheng Luo 1 , Yong Q Gu 2 , Daniela Puiu 3 , Hao Wang 4, 5, 6 , Sven O Twardziok 7 , Karin R Deal 1 , Naxin Huo 1, 2 , Tingting Zhu 1 , Le Wang 1 , Yi Wang 1, 2 , Patrick E McGuire 1 , Shuyang Liu 1 , Hai Long 1 , Ramesh K Ramasamy 1 , Juan C Rodriguez 1 , Sonny L Van 1 , Luxia Yuan 1 , Zhenzhong Wang 1, 8 , Zhiqiang Xia 1 , Lichan Xiao 1 , Olin D Anderson 2 , Shuhong Ouyang 2, 8 , Yong Liang 2, 8 , Aleksey V Zimin 3 , Geo Pertea 3 , Peng Qi 4, 5 , Jeffrey L Bennetzen 6 , Xiongtao Dai 9 , Matthew W Dawson 9 , Hans-Georg Müller 9 , Karl Kugler 7 , Lorena Rivarola-Duarte 7 , Manuel Spannagl 7 , Klaus F X Mayer 7, 10 , Fu-Hao Lu 11 , Michael W Bevan 11 , Philippe Leroy 12 , Pingchuan Li 13 , Frank M You 13 , Qixin Sun 8 , Zhiyong Liu 8 , Eric Lyons 14 , Thomas Wicker 15 , Steven L Salzberg 3, 16 , Katrien M Devos 4, 5 , Jan Dvořák 1
Affiliation  

Aegilops tauschii is the diploid progenitor of the D genome of hexaploid wheat (Triticum aestivum, genomes AABBDD) and an important genetic resource for wheat. The large size and highly repetitive nature of the Ae. tauschii genome has until now precluded the development of a reference-quality genome sequence. Here we use an array of advanced technologies, including ordered-clone genome sequencing, whole-genome shotgun sequencing, and BioNano optical genome mapping, to generate a reference-quality genome sequence for Ae. tauschii ssp. strangulata accession AL8/78, which is closely related to the wheat D genome. We show that compared to other sequenced plant genomes, including a much larger conifer genome, the Ae. tauschii genome contains unprecedented amounts of very similar repeated sequences. Our genome comparisons reveal that the Ae. tauschii genome has a greater number of dispersed duplicated genes than other sequenced genomes and its chromosomes have been structurally evolving an order of magnitude faster than those of other grass genomes. The decay of colinearity with other grass genomes correlates with recombination rates along chromosomes. We propose that the vast amounts of very similar repeated sequences cause frequent errors in recombination and lead to gene duplications and structural chromosome changes that drive fast genome evolution.

中文翻译:

小麦 D 基因组 Aegilops tauschii 祖先的基因组序列

Aegilops tauschii是六倍体小麦(Triticum aestivum,基因组AABBDD)D基因组的二倍体祖先,是小麦重要的遗传资源。Ae 的大尺寸和高度重复性。tauschii 基因组迄今为止已经排除了参考质量基因组序列的开发。在这里,我们使用一系列先进技术,包括有序克隆基因组测序、全基因组鸟枪法测序和 BioNano 光学基因组图谱,为 Ae 生成参考质量的基因组序列。tauschii ssp. strangulata 登记号 AL8/78,与小麦 D 基因组密切相关。我们表明,与其他测序的植物基因组相比,包括更大的针叶树基因组 Ae。tauschii 基因组包含前所未有的数量非常相似的重复序列。我们的基因组比较显示 Ae. tauschii 基因组具有比其他已测序基因组更多的分散重复基因,其染色体的结构进化速度比其他禾本科植物基因组快一个数量级。与其他草基因组共线性的衰减与沿染色体的重组率相关。我们提出,大量非常相似的重复序列会导致频繁的重组错误,并导致基因重复和染色体结构变化,从而推动基因组的快速进化。
更新日期:2017-11-01
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