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Dual-color quantum dots-based simultaneous detection of HPV-HIV co-infection
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2017-11-16 , DOI: 10.1016/j.snb.2017.11.074
Ana Maria Jimenez Jimenez , Amitava Moulick , Lukas Richtera , Ludmila Krejcova , Lukas Kalina , Rahul Datta , Marketa Svobodova , David Hynek , Michal Masarik , Zbynek Heger , Vojtech Adam

Human papillomavirus (HPV) and human immunodeficiency virus (HIV) are closely related infections; both diseases are mutually reinforcing due to the co-infection effects, which increase their clinical and epidemiological implications, raising urgent public health concerns. In the present study, a nano-system was developed for simultaneous detection of HPV and HIV targeting E6-HPV16 and Gag-HIV genes respectively. The target sequences from the samples of the patients suffering from head and neck cancer were isolated by magnetic glass particles (MGPs) and subsequently were hybridized with a mixture of the complementary probes attached to cadmium telluride quantum dots (CdTe QDs). Finally, the specific and successful binding was determined by an easy fluorescence analyses and was corroborated by PCR and electrochemistry. Red and green CdTe QDs were used for the dual-color detection of HPV and HIV respectively. Our nano-system can be applied as a proficient tool for the identification of HPV, HIV or both diseases simultaneously, getting a successful and high sensitivity system of detection.



中文翻译:

基于双色量子点的HPV-HIV合并感染同时检测

人乳头瘤病毒(HPV)和人免疫缺陷病毒(HIV)是密切相关的感染。两种疾病由于共同感染的影响而相互促进,这增加了它们的临床和流行病学影响,引起了紧迫的公共卫生关注。在本研究中,开发了一种纳米系统,用于同时检测针对E6-HPV16Gag-HIV的HPVHIV基因分别。通过磁性玻璃颗粒(MGP)分离患有头颈癌的患者样品的靶序列,然后将其与附着在碲化镉量子点(CdTe QD)上的互补探针混合物进行杂交。最后,通过简单的荧光分析确定了特异性和成功的结合,并通过PCR和电​​化学证实了这一点。红色和绿色CdTe QD分别用于HPV和HIV的双色检测。我们的纳米系统可以用作同时鉴定HPV,HIV或两种疾病的熟练工具,从而获得成功且高灵敏度的检测系统。

更新日期:2017-11-16
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