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Convergence of topological domain boundaries, insulators, and polytene interbands revealed by high-resolution mapping of chromatin contacts in the early Drosophila melanogaster embryo
eLife ( IF 7.7 ) Pub Date : 2017-11-17 , DOI: 10.7554/elife.29550
Michael R Stadler 1 , Jenna E Haines 1 , Michael B Eisen 1, 2, 3
Affiliation  

High-throughput assays of three-dimensional interactions of chromosomes have shed considerable light on the structure of animal chromatin. Despite this progress, the precise physical nature of observed structures and the forces that govern their establishment remain poorly understood. Here we present high resolution Hi-C data from early Drosophila embryos. We demonstrate that boundaries between topological domains of various sizes map to DNA elements that resemble classical insulator elements: short genomic regions sensitive to DNase digestion that are strongly bound by known insulator proteins and are frequently located between divergent promoters. Further, we show a striking correspondence between these elements and the locations of mapped polytene interband regions. We believe it is likely this relationship between insulators, topological boundaries, and polytene interbands extends across the genome, and we therefore propose a model in which decompaction of boundary-insulator-interband regions drives the organization of interphase chromosomes by creating stable physical separation between adjacent domains.

中文翻译:

早期果蝇胚胎染色质接触的高分辨率映射揭示的拓扑域边界、绝缘体和聚乙烯带间的收敛

染色体三维相互作用的高通量分析为动物染色质的结构提供了相当多的启示。尽管取得了这一进展,但对观察到的结构的精确物理性质以及控制其建立的力量仍然知之甚少。在这里,我们展示了来自早期果蝇胚胎的高分辨率 Hi-C 数据。我们证明了各种大小的拓扑域之间的边界映射到类似于经典绝缘子元素的 DNA 元素:对 DNase 消化敏感的短基因组区域,这些区域被已知的绝缘子蛋白强烈结合,并且经常位于不同的启动子之间。此外,我们显示了这些元素与映射的聚乙烯带间区域的位置之间的惊人对应关系。我们认为很可能是绝缘体之间的这种关系,
更新日期:2017-11-17
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