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Determination of orientin in Trollius chinensis using ultrasound-assisted extraction and high performance liquid chromatography: Several often-overlooked sample preparation parameters in an ultrasonic bath
Journal of Chromatography A ( IF 4.1 ) Pub Date : 2017-11-15 , DOI: 10.1016/j.chroma.2017.11.034
Mengxia Wei , Lei Yang

The erratic pressure intensities and cavitation exhibited in an ultrasonic bath pose various during practical application. To achieve the most efficient experimental design, this manuscript aims to discover violently sites that are subject to the effect of slight changes in position on cavitation and ultrasound intensity distributed in the ultrasonic bath. In addition, optimization several often over-looked ultrasound parameters and experimental conditions, are intended to place the reaction vessel properly and operate under suitable experimental conditions to obtain the maximum yield of target analyte. In this study, an investigation of the various ultrasound intensities and cavitation in ultrasonic bath space were conducted with the help of the orientin yield using ultrasound-assisted extraction. Conventional parameters such as the volume fraction of ethanol, liquid-solid ratio, ultrasound irradiation power, time and frequency, and reaction temperature were investigated, all of which affect the extractive yield factors. Also several often over-looked parameters such as the extraction vessel position in the ultrasonic bath base, the distance between the bottom of the extraction vessel and the ultrasonic bath base, the diameter, the shape and the texture of the extraction vessel, height of the liquid medium and the ultrasound propagating medium salt concentration in the ultrasonic bath were tested exhaustively in this study. These results can therefore serve as a guide to optimize the usage of the ultrasonic bath for future applications.



中文翻译:

超声波辅助萃取和高效液相色谱法测定金莲花中的Orientin:超声浴中几个常被忽略的样品制备参数

在实际应用中,超声波浴中表现出的不稳定的压力强度和气穴现象各不相同。为了实现最有效的实验设计,该手稿旨在发现剧烈的位置,这些位置会受到位置的轻微变化对空化和超声浴中分布的超声强度的影响。另外,优化几个经常被忽视的超声参数和实验条件,是为了正确放置反应容器并在合适的实验条件下运行以获得目标分析物的最大产率。在这项研究中,借助于超声辅助提取法,利用东方蛋白的产量,对超声浴空间中的各种超声强度和空化进行了研究。研究了常规参数,例如乙醇的体积分数,液固比,超声辐射功率,时间和频率以及反应温度,所有这些参数都会影响萃取收率。还有一些经常被忽视的参数,例如超声浴底座中的萃取容器位置,萃取容器底部与超声浴底座之间的距离,萃取容器的直径,形状和质地,容器的高度等。本研究对液体介质和超声浴中超声传播介质中的盐浓度进行了详尽的测试。因此,这些结果可以作为优化超声浴在未来应用中使用的指导。研究了反应温度和反应温度,所有这些都会影响萃取收率。还有一些经常被忽视的参数,例如超声浴底座中的萃取容器位置,萃取容器底部与超声浴底座之间的距离,萃取容器的直径,形状和质地,容器的高度等。本研究对液体介质和超声浴中超声传播介质中的盐浓度进行了详尽的测试。因此,这些结果可以作为优化超声浴在未来应用中使用的指导。研究了反应温度和反应温度,所有这些都会影响萃取收率。还有一些经常被忽视的参数,例如超声浴底座中的萃取容器位置,萃取容器底部与超声浴底座之间的距离,萃取容器的直径,形状和质地,容器的高度等。本研究对液体介质和超声浴中超声传播介质中的盐浓度进行了详尽的测试。因此,这些结果可以作为优化超声浴在未来应用中使用的指导。本研究对萃取槽底部与超声浴底部之间的距离,萃取槽的直径,形状和质地,液体介质的高度以及超声浴中超声传播介质的盐浓度进行了详尽的测试。 。因此,这些结果可以作为优化超声浴在未来应用中使用的指导。本研究对萃取槽底部与超声浴底部之间的距离,萃取槽的直径,形状和质地,液体介质的高度以及超声浴中超声传播介质的盐浓度进行了详尽的测试。 。因此,这些结果可以作为优化超声浴在未来应用中使用的指导。

更新日期:2017-11-15
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