Fluorescence polarization immunoassays (FPIA) were developed for the determination of pesticides triazophos and carbaryl in wheat grains using the portable FPIA device Sentry 200 (Ellie). FPIA of carbaryl was developed first. Tracers for FPIA were synthesized and their structures were confirmed via mass spectrometry. The influence of tracer cross-linking bridge length on the assay sensitivity was estimated. The use of tracers labeled with ethylenediamine fluorescein thiocarbamyl (EDF) resulted in the best assay sensitivity with minimal reagent consumption. A rapid and easily performed sample preparation technique that allowed the parallel determination of both analytes in aliquots of the same sample was developed. The detection limits for triazophos and carbaryl were 40 and 20 μg kg⁻¹, respectively, and the measurement ranges were 40–200 and 60–650 μg kg⁻¹, respectively. A recovery test was performed using FPIA and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), and the results obtained showed good correlation (r² = 0.9843 for triazophos, r² = 0.9916 for carbaryl).

中文翻译：

---

荧光偏振免疫分析法可同时检测小麦籽粒中农药西维因和三唑磷的开发

使用便携式FPIA设备Sentry 200（Ellie）开发了荧光偏振免疫测定法（FPIA），用于测定小麦籽粒中的农药三唑磷和西维因。首先开发了西维因的FPIA。合成了FPIA的示踪剂，并通过质谱确认了其结构。评估了示踪剂交联桥长度对测定灵敏度的影响。使用标记有乙二胺荧光素硫代氨基甲酸酯（EDF）的示踪剂可实现最佳的测定灵敏度，同时减少试剂消耗。开发了一种快速且易于执行的样品制备技术，该技术允许平行测定同一样品等分试样中的两种分析物。三唑磷和西维因的检出限分别为40和20μgkg ^-1的测量范围分别为40–200和60–650μgkg ^-1。使用FPIA和高效液相色谱-串联质谱（HPLC-MS / MS）进行了恢复测试，并且获得的结果显示出良好的相关性（三唑磷的r ² = 0.9843，西芳基的r ² = 0.9916）。